Phenolics content and antioxidant activity of wood extractives from three clones of acacia hybrid (Acacia mangium × Acacia auriculiformis)
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| Format: | Artículo científico |
| Language: | en |
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Universidad del Bío Bío
2021
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| _version_ | 1876448277544566784 |
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| author | Brandon Aristo Verick Purba |
| author_facet | Brandon Aristo Verick Purba |
| contents | Phenolics content and antioxidant activity of wood extractives from three clones of acacia hybrid (Acacia mangium × Acacia auriculiformis) Brandon Aristo Verick Purba Sri Sunarti Ganis Lukmandaru Agrociencias sapwood heartwood Acacia hybrid wood extractives phenolic contents Breeding strategy of Acacia hybrid is being developed by Center for Forest Biotechnology and Tree Improvement Indonesia and has produced three superior clones in growth (Clone 16, 25, and 44). Understanding the extractives of the new clones might determine future development steps to improve its resistance to diseases especially heartrot. The objective of this study was to investigate the extractive content in three radial directions (SW = sapwood; OHW = outer heartwood; IHW = inner heartwood); total phenolic, flavonoid, flavanol contents (colorimetric assay); and antioxidant activity (1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay). Extractions were done with three different solvents in successive (n-hexane = H; methanol = M; hot water = W), yielded 0,69 % - 1,70 %; 1,51 % - 10,86 %; and 0,51 % - 1,16 % of extractive contents, respectively. The total phenolic content (TPC) from TPC-H, TPC-M, and TPC-W ranged between 3,68 mg of gallic acid equivalent (GAE)/g - 10,41 mg GAE/g; 76,83 mg GAE/g - 448,35 mg GAE/g; and 43,28 mg GAE/g - 198,92 mg GAE/g, respectively; the total flavonoid content (TFC) from TFC-H, TFC-M, and TFC-W between 4,23 mg of quercetin equivalent (QE)/g - 41,51 mg QE/g; 29,55 mg QE/g - 133,71 mg QE/g; and 7,70 mg QE/g - 29,37 mg QE/g, respectively; total flavanol content (TVC) from TVC-H, TVC-M, and TVC-W ranged between 28,74 mg of catechin equivalent (CE)/g - 66,90 mg CE/g; 83,39 mg CE/g - 247,18 mg CE/g; and 7,08 mg CE/g - 29,21 mg CE/g, respectively. Furthermore, the antioxidant activity was found to be significantly affected by the radial factor with the strongest activity exhibited by inner heartwood extract with an IC50 value of 255,77 μg/ml (gallic acid IC50 showed a value of 39,00 μg/ml). Among clones, clone 16 was determined to have the highest extractive, total flavonoid as well as flavanol contents. Thus, clone 16 was hypothesized to be more resistance against heart rot disease. 2021 artículo científico 0717-3644 https://www.redalyc.org/articulo.oa?id=48565417031 https://www.redalyc.org/journal/485/48565417031/ https://www.redalyc.org/journal/485/48565417031/html/ https://www.redalyc.org/journal/485/48565417031/48565417031.epub https://www.redalyc.org/journal/485/48565417031/movil en http://www.redalyc.org/revista.oa?id=485 Maderas. Ciencia y Tecnología application/pdf Universidad del Bío Bío Maderas. Ciencia y Tecnología (Chile) Vol.23 |
| format | Artículo científico |
| id | redalyc_48565417031 |
| institution | Redalyc |
| language | en |
| publishDate | 2021 |
| publisher | Universidad del Bío Bío |
| spellingShingle | Phenolics content and antioxidant activity of wood extractives from three clones of acacia hybrid (Acacia mangium × Acacia auriculiformis) Brandon Aristo Verick Purba Agrociencias sapwood heartwood Acacia hybrid wood extractives phenolic contents Phenolics content and antioxidant activity of wood extractives from three clones of acacia hybrid (Acacia mangium × Acacia auriculiformis) Brandon Aristo Verick Purba Sri Sunarti Ganis Lukmandaru Agrociencias sapwood heartwood Acacia hybrid wood extractives phenolic contents Breeding strategy of Acacia hybrid is being developed by Center for Forest Biotechnology and Tree Improvement Indonesia and has produced three superior clones in growth (Clone 16, 25, and 44). Understanding the extractives of the new clones might determine future development steps to improve its resistance to diseases especially heartrot. The objective of this study was to investigate the extractive content in three radial directions (SW = sapwood; OHW = outer heartwood; IHW = inner heartwood); total phenolic, flavonoid, flavanol contents (colorimetric assay); and antioxidant activity (1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay). Extractions were done with three different solvents in successive (n-hexane = H; methanol = M; hot water = W), yielded 0,69 % - 1,70 %; 1,51 % - 10,86 %; and 0,51 % - 1,16 % of extractive contents, respectively. The total phenolic content (TPC) from TPC-H, TPC-M, and TPC-W ranged between 3,68 mg of gallic acid equivalent (GAE)/g - 10,41 mg GAE/g; 76,83 mg GAE/g - 448,35 mg GAE/g; and 43,28 mg GAE/g - 198,92 mg GAE/g, respectively; the total flavonoid content (TFC) from TFC-H, TFC-M, and TFC-W between 4,23 mg of quercetin equivalent (QE)/g - 41,51 mg QE/g; 29,55 mg QE/g - 133,71 mg QE/g; and 7,70 mg QE/g - 29,37 mg QE/g, respectively; total flavanol content (TVC) from TVC-H, TVC-M, and TVC-W ranged between 28,74 mg of catechin equivalent (CE)/g - 66,90 mg CE/g; 83,39 mg CE/g - 247,18 mg CE/g; and 7,08 mg CE/g - 29,21 mg CE/g, respectively. Furthermore, the antioxidant activity was found to be significantly affected by the radial factor with the strongest activity exhibited by inner heartwood extract with an IC50 value of 255,77 μg/ml (gallic acid IC50 showed a value of 39,00 μg/ml). Among clones, clone 16 was determined to have the highest extractive, total flavonoid as well as flavanol contents. Thus, clone 16 was hypothesized to be more resistance against heart rot disease. 2021 artículo científico 0717-3644 https://www.redalyc.org/articulo.oa?id=48565417031 https://www.redalyc.org/journal/485/48565417031/ https://www.redalyc.org/journal/485/48565417031/html/ https://www.redalyc.org/journal/485/48565417031/48565417031.epub https://www.redalyc.org/journal/485/48565417031/movil en http://www.redalyc.org/revista.oa?id=485 Maderas. Ciencia y Tecnología application/pdf Universidad del Bío Bío Maderas. Ciencia y Tecnología (Chile) Vol.23 |
| title | Phenolics content and antioxidant activity of wood extractives from three clones of acacia hybrid (Acacia mangium × Acacia auriculiformis) |
| topic | Agrociencias sapwood heartwood Acacia hybrid wood extractives phenolic contents |
| url | https://www.redalyc.org/articulo.oa?id=48565417031 https://www.redalyc.org/journal/485/48565417031/ https://www.redalyc.org/journal/485/48565417031/html/ https://www.redalyc.org/journal/485/48565417031/48565417031.epub https://www.redalyc.org/journal/485/48565417031/movil |