Cellulose biosaccharification by Irpex lacteus wood decay fungus
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad del Bío Bío
2023
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| _version_ | 1876477959909408768 |
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| author | Sergiy Boiko |
| author_facet | Sergiy Boiko |
| contents | Cellulose biosaccharification by Irpex lacteus wood decay fungus Sergiy Boiko Maksym Netsvetov Vladimir Radchenko Agrociencias exo endo Cellulose glucanases Irpex lacteus Enzymatic hydrolysis is an environmentally friendly technology to produce sugars from pretreated biomass. Here, we show that the new Il-11 Irpex lacteus strain can synthesize cellulases in a high quantity. The peptone and filter paper contained in the medium significantly enhanced activity of endo-1,4-β-D-glucanases (app. 50 IU/mL) and total cellulases (app. 9 IU/mL), whereas the medium with peptone and sodium carboxymethyl cellulose stimulated activity of exo-1,4-β-D-glucanases (33 IU/mL). The expression of cellulases reached its maximum within 96-144 hours, and the optimum pH is 3,7. Thermal treatment at 30 °C for 60 minutes activated endo-1,4-β-D-glucanases and total cellulases, while exo-1,4-β-D-glucanases activity was enhanced following 40 °C treatment. In total, the cellulases complex (300 IU/g) saccharified untreated cellulose by 38 % in 48 hours. Concentrate with filter paper activity 100 IU/g is the more balanced enzyme-substrate ratio (2 %), which allows prolonging the saccharification process that will have a positive effect on the cost of the final product. 2023 artículo científico 0717-3644 https://www.redalyc.org/articulo.oa?id=48575202035 https://www.redalyc.org/journal/485/48575202035/ https://www.redalyc.org/journal/485/48575202035/html/ https://www.redalyc.org/journal/485/48575202035/48575202035.epub https://www.redalyc.org/journal/485/48575202035/movil 10.4067/s0718-221x2023000100435 en http://www.redalyc.org/revista.oa?id=485 Maderas. Ciencia y Tecnología application/pdf Universidad del Bío Bío Maderas. Ciencia y Tecnología (Chile) Vol.25 |
| format | Artículo científico |
| id | redalyc_48575202035 |
| institution | Redalyc |
| language | en |
| publishDate | 2023 |
| publisher | Universidad del Bío Bío |
| spellingShingle | Cellulose biosaccharification by Irpex lacteus wood decay fungus Sergiy Boiko Agrociencias exo endo Cellulose glucanases Irpex lacteus Cellulose biosaccharification by Irpex lacteus wood decay fungus Sergiy Boiko Maksym Netsvetov Vladimir Radchenko Agrociencias exo endo Cellulose glucanases Irpex lacteus Enzymatic hydrolysis is an environmentally friendly technology to produce sugars from pretreated biomass. Here, we show that the new Il-11 Irpex lacteus strain can synthesize cellulases in a high quantity. The peptone and filter paper contained in the medium significantly enhanced activity of endo-1,4-β-D-glucanases (app. 50 IU/mL) and total cellulases (app. 9 IU/mL), whereas the medium with peptone and sodium carboxymethyl cellulose stimulated activity of exo-1,4-β-D-glucanases (33 IU/mL). The expression of cellulases reached its maximum within 96-144 hours, and the optimum pH is 3,7. Thermal treatment at 30 °C for 60 minutes activated endo-1,4-β-D-glucanases and total cellulases, while exo-1,4-β-D-glucanases activity was enhanced following 40 °C treatment. In total, the cellulases complex (300 IU/g) saccharified untreated cellulose by 38 % in 48 hours. Concentrate with filter paper activity 100 IU/g is the more balanced enzyme-substrate ratio (2 %), which allows prolonging the saccharification process that will have a positive effect on the cost of the final product. 2023 artículo científico 0717-3644 https://www.redalyc.org/articulo.oa?id=48575202035 https://www.redalyc.org/journal/485/48575202035/ https://www.redalyc.org/journal/485/48575202035/html/ https://www.redalyc.org/journal/485/48575202035/48575202035.epub https://www.redalyc.org/journal/485/48575202035/movil 10.4067/s0718-221x2023000100435 en http://www.redalyc.org/revista.oa?id=485 Maderas. Ciencia y Tecnología application/pdf Universidad del Bío Bío Maderas. Ciencia y Tecnología (Chile) Vol.25 |
| title | Cellulose biosaccharification by Irpex lacteus wood decay fungus |
| topic | Agrociencias exo endo Cellulose glucanases Irpex lacteus |
| url | https://www.redalyc.org/articulo.oa?id=48575202035 https://www.redalyc.org/journal/485/48575202035/ https://www.redalyc.org/journal/485/48575202035/html/ https://www.redalyc.org/journal/485/48575202035/48575202035.epub https://www.redalyc.org/journal/485/48575202035/movil |