ULTRASTRUCTURAL CALLI ANALYSIS OF Inga vera WILLD. SUBSP. Affinis (DC.) T.D. PENN
Fuente:
Redalyc
Salvato in:
| Autore principale: | |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Universidade Federal de Viçosa
2010
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1876483975501840384 |
|---|---|
| author | Vanessa Cristina Stein |
| author_facet | Vanessa Cristina Stein |
| contents | ULTRASTRUCTURAL CALLI ANALYSIS OF Inga vera WILLD. SUBSP. Affinis (DC.) T.D. PENN Vanessa Cristina Stein Renato Paiva Daiane Peixoto Vargas Fernanda Pereira Soares Eduardo Alves Gabriela Ferreira Nogueira Agrociencias Anthers Ovaries Nodal segments Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin was used, and for the callogenesis of anthers and ovaries, where the culture medium was enriched with 0.25% activated charcoal and 90µM PVP. After 45 days in culture medium, the anther, ovary, leaf and nodal segment calli were fixed in Karnovisky and prepared for visualization by scanning and transmission electron microscopy. Ultrastructural differences were observed among the callus cells of anthers, ovaries, segments and leaves. There was no evidence of somatic embryo formation in the anther, leaf and nodal segment calli, in spite of some embryogenic characteristics in the cells. The ovary calli, with indications of embryo formation, seem to be the most responsive explant source for embryogenesis. 2010 artículo científico 0100-6762 https://www.redalyc.org/articulo.oa?id=48815860004 en http://www.redalyc.org/revista.oa?id=488 Revista Árvore application/pdf Universidade Federal de Viçosa Revista Árvore (Brasil) Num.5 Vol.34 |
| format | Artículo científico |
| id | redalyc_48815860004 |
| institution | Redalyc |
| language | en |
| publishDate | 2010 |
| publisher | Universidade Federal de Viçosa |
| spellingShingle | ULTRASTRUCTURAL CALLI ANALYSIS OF Inga vera WILLD. SUBSP. Affinis (DC.) T.D. PENN Vanessa Cristina Stein Agrociencias Anthers Ovaries Nodal segments ULTRASTRUCTURAL CALLI ANALYSIS OF Inga vera WILLD. SUBSP. Affinis (DC.) T.D. PENN Vanessa Cristina Stein Renato Paiva Daiane Peixoto Vargas Fernanda Pereira Soares Eduardo Alves Gabriela Ferreira Nogueira Agrociencias Anthers Ovaries Nodal segments Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin was used, and for the callogenesis of anthers and ovaries, where the culture medium was enriched with 0.25% activated charcoal and 90µM PVP. After 45 days in culture medium, the anther, ovary, leaf and nodal segment calli were fixed in Karnovisky and prepared for visualization by scanning and transmission electron microscopy. Ultrastructural differences were observed among the callus cells of anthers, ovaries, segments and leaves. There was no evidence of somatic embryo formation in the anther, leaf and nodal segment calli, in spite of some embryogenic characteristics in the cells. The ovary calli, with indications of embryo formation, seem to be the most responsive explant source for embryogenesis. 2010 artículo científico 0100-6762 https://www.redalyc.org/articulo.oa?id=48815860004 en http://www.redalyc.org/revista.oa?id=488 Revista Árvore application/pdf Universidade Federal de Viçosa Revista Árvore (Brasil) Num.5 Vol.34 |
| title | ULTRASTRUCTURAL CALLI ANALYSIS OF Inga vera WILLD. SUBSP. Affinis (DC.) T.D. PENN |
| topic | Agrociencias Anthers Ovaries Nodal segments |
| url | https://www.redalyc.org/articulo.oa?id=48815860004 |