In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae)
Fuente:
Redalyc
Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidade Federal de Viçosa
2003
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1876472322013003776 |
|---|---|
| author | Vanda Marilza de Carvalho |
| author_facet | Vanda Marilza de Carvalho |
| contents | In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) Vanda Marilza de Carvalho Maria de Fátima Machado Pires da Silva Arildo José Oliveira Braz de Alexandre Ferreira Fernando Y. Sato Agrociencias Apocynaceae Micropropagation and tissue culture Tabernaemontana fuchsiaefolia This study describes a simple and promising for in vitro multiplication of Tabernaemontanafuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source ofcompounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MSmedium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine(kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation.Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetininduced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateralbuds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested.Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings.The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferredto MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA 2003 artículo científico 0100-6762 https://www.redalyc.org/articulo.oa?id=48827401 en http://www.redalyc.org/revista.oa?id=488 Revista Árvore application/pdf Universidade Federal de Viçosa Revista Árvore (Brasil) Num.4 Vol.27 |
| format | Artículo científico |
| id | redalyc_48827401 |
| institution | Redalyc |
| language | en |
| publishDate | 2003 |
| publisher | Universidade Federal de Viçosa |
| spellingShingle | In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) Vanda Marilza de Carvalho Agrociencias Apocynaceae Micropropagation and tissue culture Tabernaemontana fuchsiaefolia In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) Vanda Marilza de Carvalho Maria de Fátima Machado Pires da Silva Arildo José Oliveira Braz de Alexandre Ferreira Fernando Y. Sato Agrociencias Apocynaceae Micropropagation and tissue culture Tabernaemontana fuchsiaefolia This study describes a simple and promising for in vitro multiplication of Tabernaemontanafuchsiaefolia, a species abundantly found in southern Brazil utilized for medicinal purposes and as a source ofcompounds that may be used to develop new synthetic drugs. Apical and hypocotyl explants were cultured in MSmedium containing different concentrations of the cytokinins benzylaminopurine (BA) and 6-furfurylaminopurine(kinetin), supplemented with phloroglucinol (1, 3, 5-hydroxybenzene) to stimulate growth and shoot proliferation.Cytokinin added to the culture media positively influenced the micropropagation of T. fuchsiaefolia.and kinetininduced more shoots per explant than BA cytokinin. A favorable effect of phloroglucinol on apical and lateralbuds from hypocotyls was also achieved in medium containing no kinetin or in all kinetin concentrations tested.Short pulses of auxin 3-indolebutyric acid (IBA) 5.0 mg/l resulted in satisfactory rooting in apical microcuttings.The addition of phloroglucinol to MS medium induced rhizogenesis in 29% of the nodal segments transferredto MS medium in the absence of IBA and in 50% of the nodal segments transferred to MS medium containing0.5 mg/l IBA and in nodal segments previously submitted to short pulses of IBA 2003 artículo científico 0100-6762 https://www.redalyc.org/articulo.oa?id=48827401 en http://www.redalyc.org/revista.oa?id=488 Revista Árvore application/pdf Universidade Federal de Viçosa Revista Árvore (Brasil) Num.4 Vol.27 |
| title | In vitro multiplication of Tabernaemontana fuchsiaefolia L. (Apocynaceae) |
| topic | Agrociencias Apocynaceae Micropropagation and tissue culture Tabernaemontana fuchsiaefolia |
| url | https://www.redalyc.org/articulo.oa?id=48827401 |