GENETIC TRANSFORMATION OF Eucalyptus camaldulensis BY AGROBALISTIC METHOD
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| Format: | Artículo científico |
| Language: | en |
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Universidade Federal de Viçosa
2013
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| author | Evânia Galvão Mendonça |
| author_facet | Evânia Galvão Mendonça |
| contents | GENETIC TRANSFORMATION OF Eucalyptus camaldulensis BY AGROBALISTIC METHOD Evânia Galvão Mendonça Vanessa Cristina Stein Flávia Pereira Balieiro Carolina Delfin Fernandes Lima Breno Régis Santos Luciano Vilela Paiva Agrociencias pCambia 3301 Tissue culture tumefasciens EHA 105 Transient expression Eucalyptus stands in the setting of worldwide forestry due to its adaptability, rapid growth, production of high-quality and low cost of wood pulp fibers. The eucalyptus convetional breeding is impaired mainlly by the long life cycle making the genetic transformation systems an important tool for this purpose. However, this system requires in vitro eficient protocols for plant induction, regeneration and seletion, that allow to obtain transgenic plants from the transformed cell groups. The aim of this work was to evaluate the callus formation and to optimize the leaves and callus genetic transformation protocol by using the Agrobacterium tumefaciens system. Concerning callus formation, two different culture media were evaluated: MS medium supplemented with auxin, cytokinin (M1) and the MS medium with reduced nitrogen concentration and supplemented with auxin, cytokinin coconut water (M2). To establish the leave genetic transformation, those were exposed to agrobiolistics technique (gene gun), to tissue injury, and A. tumesfasciens EHA 105 contening the vetor pCambia 3301 (35S::GUS::NOS), for gene transference and to establish the callus transformation thoses were exposed only to A. tumefasciens. For both experiments, the influence of different infection periods was evaluated. The M2 medium provided the best values for callus sizea and fresh and dry weight. The leaves genetic transformation using the agrobiolistics technique was effective, the gus gene transient expression could be observed. No significant differences were obtained in the infection periods (4, 6 and 8 minutes). The callus genetic transformation with A. tumefaciens also promotend the gus gene transient expression on the callus co-cultiveted for 15 e 30 minutes. The transformed callus was transfered to a regeneration and selection medium and transformed plants were obtained. 2013 artículo científico 0100-6762 https://www.redalyc.org/articulo.oa?id=48828116005 en http://www.redalyc.org/revista.oa?id=488 Revista Árvore application/pdf Universidade Federal de Viçosa Revista Árvore (Brasil) Num.3 Vol.37 |
| format | Artículo científico |
| id | redalyc_48828116005 |
| institution | Redalyc |
| language | en |
| publishDate | 2013 |
| publisher | Universidade Federal de Viçosa |
| spellingShingle | GENETIC TRANSFORMATION OF Eucalyptus camaldulensis BY AGROBALISTIC METHOD Evânia Galvão Mendonça Agrociencias pCambia 3301 Tissue culture tumefasciens EHA 105 Transient expression GENETIC TRANSFORMATION OF Eucalyptus camaldulensis BY AGROBALISTIC METHOD Evânia Galvão Mendonça Vanessa Cristina Stein Flávia Pereira Balieiro Carolina Delfin Fernandes Lima Breno Régis Santos Luciano Vilela Paiva Agrociencias pCambia 3301 Tissue culture tumefasciens EHA 105 Transient expression Eucalyptus stands in the setting of worldwide forestry due to its adaptability, rapid growth, production of high-quality and low cost of wood pulp fibers. The eucalyptus convetional breeding is impaired mainlly by the long life cycle making the genetic transformation systems an important tool for this purpose. However, this system requires in vitro eficient protocols for plant induction, regeneration and seletion, that allow to obtain transgenic plants from the transformed cell groups. The aim of this work was to evaluate the callus formation and to optimize the leaves and callus genetic transformation protocol by using the Agrobacterium tumefaciens system. Concerning callus formation, two different culture media were evaluated: MS medium supplemented with auxin, cytokinin (M1) and the MS medium with reduced nitrogen concentration and supplemented with auxin, cytokinin coconut water (M2). To establish the leave genetic transformation, those were exposed to agrobiolistics technique (gene gun), to tissue injury, and A. tumesfasciens EHA 105 contening the vetor pCambia 3301 (35S::GUS::NOS), for gene transference and to establish the callus transformation thoses were exposed only to A. tumefasciens. For both experiments, the influence of different infection periods was evaluated. The M2 medium provided the best values for callus sizea and fresh and dry weight. The leaves genetic transformation using the agrobiolistics technique was effective, the gus gene transient expression could be observed. No significant differences were obtained in the infection periods (4, 6 and 8 minutes). The callus genetic transformation with A. tumefaciens also promotend the gus gene transient expression on the callus co-cultiveted for 15 e 30 minutes. The transformed callus was transfered to a regeneration and selection medium and transformed plants were obtained. 2013 artículo científico 0100-6762 https://www.redalyc.org/articulo.oa?id=48828116005 en http://www.redalyc.org/revista.oa?id=488 Revista Árvore application/pdf Universidade Federal de Viçosa Revista Árvore (Brasil) Num.3 Vol.37 |
| title | GENETIC TRANSFORMATION OF Eucalyptus camaldulensis BY AGROBALISTIC METHOD |
| topic | Agrociencias pCambia 3301 Tissue culture tumefasciens EHA 105 Transient expression |
| url | https://www.redalyc.org/articulo.oa?id=48828116005 |