MICROPROPAGATION AND ACCLIMATIZATION OF Aegiphila verticillata Vell.: AN ENDANGERED WOODY SPECIES
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Universidade Federal de Viçosa
2015
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| author | Luísa Maria Silveira de Almeida |
| author_facet | Luísa Maria Silveira de Almeida |
| contents | MICROPROPAGATION AND ACCLIMATIZATION OF Aegiphila verticillata Vell.: AN ENDANGERED WOODY SPECIES Luísa Maria Silveira de Almeida Leandro Elias Morais Cristiano Ferrara de Resende Virgínia Fernandes Braga Paula da Fonseca Pereira Rodolpho Abrantes Camerini e Silva Paulo Henrique Pereira Peixoto Agrociencias Rocky fields Ex vitro culture In vitro propagation The objective of this work was to establish an efficient protocol for in vitro multiplication and rooting, as well as ex vitro acclimatization of Aegiphila verticillata, a woody species found in Brazilian rocky fields. Aseptic cultures were established by seeds and two multiplication analyses were performed. In the first, we employed 6-benzylaminopurine (BAP – 0, 2.5, 5 and 7.5 μM) + α-naphthalene acetic acid (NAA – 0, 0.2, 0.4 and 0.6 μM) and, in the second, were studied adenine sulfate, kinetin and thidiazuron (0, 5, 7.5, 10 and 12.5 μM). After 90 days, we assessed the quantitative and qualitative shoot propagation. There were more than 90% seed germination and low contamination (2%). In multiplication phase, the culture medium that promoted the best quantitative and qualitative culture development was supplemented with 7.5 μM BAP + 0.4 μM NAA. In the rooting assay, were used NAA, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) (0, 0.1, 0.2, 0.3 or 0.4 μM). After 90 days, the root number and rooting quality were evaluated. In this analysis, differences were not found between the control and the other treatments. Rooted plantlets were acclimatized in styrofoam trays for 30 days, after which they were transferred to pots in the greenhouse. Only 3% of the plants subjected to initial acclimatization died and 70% of the plants transferred to the field conditions survived and showed normal development. The results founded in this work are the first involving in vitro propagation and ex vitro acclimatization of Aegiphila verticillata and provide a continuous supply of this medicinal native species, endangered due anthropogenic activities. 2015 artículo científico 0100-6762 https://www.redalyc.org/articulo.oa?id=48839479010 en http://www.redalyc.org/revista.oa?id=488 Revista Árvore application/pdf Universidade Federal de Viçosa Revista Árvore (Brasil) Num.2 Vol.39 |
| format | Artículo científico |
| id | redalyc_48839479010 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidade Federal de Viçosa |
| spellingShingle | MICROPROPAGATION AND ACCLIMATIZATION OF Aegiphila verticillata Vell.: AN ENDANGERED WOODY SPECIES Luísa Maria Silveira de Almeida Agrociencias Rocky fields Ex vitro culture In vitro propagation MICROPROPAGATION AND ACCLIMATIZATION OF Aegiphila verticillata Vell.: AN ENDANGERED WOODY SPECIES Luísa Maria Silveira de Almeida Leandro Elias Morais Cristiano Ferrara de Resende Virgínia Fernandes Braga Paula da Fonseca Pereira Rodolpho Abrantes Camerini e Silva Paulo Henrique Pereira Peixoto Agrociencias Rocky fields Ex vitro culture In vitro propagation The objective of this work was to establish an efficient protocol for in vitro multiplication and rooting, as well as ex vitro acclimatization of Aegiphila verticillata, a woody species found in Brazilian rocky fields. Aseptic cultures were established by seeds and two multiplication analyses were performed. In the first, we employed 6-benzylaminopurine (BAP – 0, 2.5, 5 and 7.5 μM) + α-naphthalene acetic acid (NAA – 0, 0.2, 0.4 and 0.6 μM) and, in the second, were studied adenine sulfate, kinetin and thidiazuron (0, 5, 7.5, 10 and 12.5 μM). After 90 days, we assessed the quantitative and qualitative shoot propagation. There were more than 90% seed germination and low contamination (2%). In multiplication phase, the culture medium that promoted the best quantitative and qualitative culture development was supplemented with 7.5 μM BAP + 0.4 μM NAA. In the rooting assay, were used NAA, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) (0, 0.1, 0.2, 0.3 or 0.4 μM). After 90 days, the root number and rooting quality were evaluated. In this analysis, differences were not found between the control and the other treatments. Rooted plantlets were acclimatized in styrofoam trays for 30 days, after which they were transferred to pots in the greenhouse. Only 3% of the plants subjected to initial acclimatization died and 70% of the plants transferred to the field conditions survived and showed normal development. The results founded in this work are the first involving in vitro propagation and ex vitro acclimatization of Aegiphila verticillata and provide a continuous supply of this medicinal native species, endangered due anthropogenic activities. 2015 artículo científico 0100-6762 https://www.redalyc.org/articulo.oa?id=48839479010 en http://www.redalyc.org/revista.oa?id=488 Revista Árvore application/pdf Universidade Federal de Viçosa Revista Árvore (Brasil) Num.2 Vol.39 |
| title | MICROPROPAGATION AND ACCLIMATIZATION OF Aegiphila verticillata Vell.: AN ENDANGERED WOODY SPECIES |
| topic | Agrociencias Rocky fields Ex vitro culture In vitro propagation |
| url | https://www.redalyc.org/articulo.oa?id=48839479010 |