SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CASSAVA STEMS
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| Format: | Artículo científico |
| Language: | en |
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Universidad Nacional de Colombia
2013
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| author | HADER CASTAÑO PELÁEZ |
| author_facet | HADER CASTAÑO PELÁEZ |
| contents | SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CASSAVA STEMS HADER CASTAÑO PELÁEZ JUAN REALES ALFARO JOSÉ ZAPATA MONTOYA Ingeniería Ethanol Fermentation Optimization Cassava Stems Saccharifi cation This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained throughthe simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination andvalidation of the optimal conditions for and the evaluation of the process of ethanol production in a bioreactor. Alkaline-pretreated cassava stemswere used as the substrate in a solid to liquid ratio of 1:10; the enzymatic complex Accellerase 1500 and the yeast Ethanol Red were evaluatedat two levels at a temperature of 38° C and a pH of 4.0 in an Erlenmeyer fl ask. The following were evaluated as process controls: simultaneoussaccharifi cation and fermentation of non-pretreated stems and separate saccharifi cation and fermentation of pretreated stems. A regression analysiswas conducted, and the resulting model was maximized using genetic algorithms. At the optimal conditions identifi ed in an Erlenmeyer fl ask, theproduction of ethanol in a 5-liter bioreactor was subsequently evaluated. An experimental concentration of ethanol of 1.88±0.04% v/v (1.99%v/v simulated optimum) was obtained using an inoculum concentration of 1.59 g/L and an enzyme concentration of 13.3 FPU/g. This value wasapproximately four times the quantity of ethanol produced without pretreatment or by the separate saccharifi cation and fermentation of pretreatedcassava stems. The evaluation of the process in the bioreactor yielded an ethanol concentration 20% less than that reached in the Erlenmeyer fl ask. 2013 artículo científico 0012-7353 https://www.redalyc.org/articulo.oa?id=49627455014 en http://www.redalyc.org/revista.oa?id=496 Dyna application/pdf Universidad Nacional de Colombia Dyna (Colombia) Num.180 Vol.80 |
| format | Artículo científico |
| id | redalyc_49627455014 |
| institution | Redalyc |
| language | en |
| publishDate | 2013 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CASSAVA STEMS HADER CASTAÑO PELÁEZ Ingeniería Ethanol Fermentation Optimization Cassava Stems Saccharifi cation SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CASSAVA STEMS HADER CASTAÑO PELÁEZ JUAN REALES ALFARO JOSÉ ZAPATA MONTOYA Ingeniería Ethanol Fermentation Optimization Cassava Stems Saccharifi cation This research evaluates the effects of the inoculum size and enzymatic activity on the concentration of ethanol obtained throughthe simultaneous saccharifi cation and fermentation of alkali-pretreated cassava stems. Other goals for this study include the determination andvalidation of the optimal conditions for and the evaluation of the process of ethanol production in a bioreactor. Alkaline-pretreated cassava stemswere used as the substrate in a solid to liquid ratio of 1:10; the enzymatic complex Accellerase 1500 and the yeast Ethanol Red were evaluatedat two levels at a temperature of 38° C and a pH of 4.0 in an Erlenmeyer fl ask. The following were evaluated as process controls: simultaneoussaccharifi cation and fermentation of non-pretreated stems and separate saccharifi cation and fermentation of pretreated stems. A regression analysiswas conducted, and the resulting model was maximized using genetic algorithms. At the optimal conditions identifi ed in an Erlenmeyer fl ask, theproduction of ethanol in a 5-liter bioreactor was subsequently evaluated. An experimental concentration of ethanol of 1.88±0.04% v/v (1.99%v/v simulated optimum) was obtained using an inoculum concentration of 1.59 g/L and an enzyme concentration of 13.3 FPU/g. This value wasapproximately four times the quantity of ethanol produced without pretreatment or by the separate saccharifi cation and fermentation of pretreatedcassava stems. The evaluation of the process in the bioreactor yielded an ethanol concentration 20% less than that reached in the Erlenmeyer fl ask. 2013 artículo científico 0012-7353 https://www.redalyc.org/articulo.oa?id=49627455014 en http://www.redalyc.org/revista.oa?id=496 Dyna application/pdf Universidad Nacional de Colombia Dyna (Colombia) Num.180 Vol.80 |
| title | SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CASSAVA STEMS |
| topic | Ingeniería Ethanol Fermentation Optimization Cassava Stems Saccharifi cation |
| url | https://www.redalyc.org/articulo.oa?id=49627455014 |