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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad Nacional de Colombia
2018
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| Online Access: | https://www.redalyc.org/articulo.oa?id=49657889014 https://www.redalyc.org/journal/496/49657889014/ https://www.redalyc.org/journal/496/49657889014/html/ https://www.redalyc.org/journal/496/49657889014/49657889014.epub https://www.redalyc.org/journal/496/49657889014/movil |
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Table of Contents:
- Streptomyces clavuligerus strain selection for clavulanic acid biosynthesis: a study based on culture composition effects and statistical analysis Laura Pinilla León F. Toro Claudio Avignone Rossa Mariana Peñuela Rigoberto Ríos Estepa Ingeniería antibiotics Clavulanic acid strain selection culture media design Streptomyces clavuligerus The clinical and industrial importance of clavulanic acid (CA) promotes the search for novel strategies to boost productivity at lower costs. In this contribution, the production of CA was evaluated in two reference strains of Streptomyces clavuligerus (Sc), ATCC27064 and DSM41826; the strains were tested in different culture media, using product yields and culture media costs as selection criteria. On average, Sc ATCC reached a concentration of CA 1.6-fold higher than Sc DSM, using the isolated soybean protein (ISP) culture medium. A central composite experimental design was used to optimize the medium, by studying the influence of ISP and K2HPO4 concentration on the production of CA. The maximum CA concentration obtained in this optimized medium was 56 mg·L-1, close to the theoretical optimum. Our results confirm that a culture medium containing a rich carbon source such as glycerol and/or starch provides the best conditions for CA production. 2018 artículo científico 0012-7353 https://www.redalyc.org/articulo.oa?id=49657889014 https://www.redalyc.org/journal/496/49657889014/ https://www.redalyc.org/journal/496/49657889014/html/ https://www.redalyc.org/journal/496/49657889014/49657889014.epub https://www.redalyc.org/journal/496/49657889014/movil 10.15446/dyna.v85n205.69560 en http://www.redalyc.org/revista.oa?id=496 Dyna application/pdf Universidad Nacional de Colombia Dyna (Colombia) Num.205 Vol.85