Determination of citric and oxalic acid in fungi fermentation broth through HPLC-DAD and solid-phase extraction
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| Format: | Artículo científico |
| Langue: | en |
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Universidad Nacional de Colombia
2020
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| _version_ | 1876443106958639104 |
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| author | Laura Trujillo-Vargas |
| author_facet | Laura Trujillo-Vargas |
| contents | Determination of citric and oxalic acid in fungi fermentation broth through HPLC-DAD and solid-phase extraction Laura Trujillo-Vargas Margarita Enid Ramírez-Carmona Yesid Vélez-Salazar Ingeniería HPLC citric acid oxalic acid solid phase extraction fermentation with fungi This work describes a method for the simultaneous determination of organic acids in fungi fermentation broths. The sample preparation technique and the chromatographic conditions were optimized to enable the monitoring of the fermentation process. The determination of the citric and oxalic acid was done with a high resolution liquid chromatograph adapted to a detector with diode arrangement, an IC-Pak Ion-Exclusion 7µm (7.8 x 300 mm) column and a mobile phase of 0.001N H2SO4 with isocratic elusion. The validation parameters show efficiency, adequate linearity, and standard deviation values (%RSD) from 0.018% and 4.650%. the quantification limits (LDC) were 50.76 mg/L for citric acid and 20.18 mg/L for oxalic acid and the detection limits (LDD) were 0.6 mg/L for both acids. The method was applied in the analysis of organic acids in fermentation broths of Aspergillus niger. 2020 artículo científico 0012-7353 https://www.redalyc.org/articulo.oa?id=49663642003 https://www.redalyc.org/journal/496/49663642003/ https://www.redalyc.org/journal/496/49663642003/html/ https://www.redalyc.org/journal/496/49663642003/49663642003.epub https://www.redalyc.org/journal/496/49663642003/movil 10.15446/dyna.v87n212.76240 en http://www.redalyc.org/revista.oa?id=496 Dyna application/pdf Universidad Nacional de Colombia Dyna (Colombia) Num.212 Vol.87 |
| format | Artículo científico |
| id | redalyc_49663642003 |
| institution | Redalyc |
| language | en |
| publishDate | 2020 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Determination of citric and oxalic acid in fungi fermentation broth through HPLC-DAD and solid-phase extraction Laura Trujillo-Vargas Ingeniería HPLC citric acid oxalic acid solid phase extraction fermentation with fungi Determination of citric and oxalic acid in fungi fermentation broth through HPLC-DAD and solid-phase extraction Laura Trujillo-Vargas Margarita Enid Ramírez-Carmona Yesid Vélez-Salazar Ingeniería HPLC citric acid oxalic acid solid phase extraction fermentation with fungi This work describes a method for the simultaneous determination of organic acids in fungi fermentation broths. The sample preparation technique and the chromatographic conditions were optimized to enable the monitoring of the fermentation process. The determination of the citric and oxalic acid was done with a high resolution liquid chromatograph adapted to a detector with diode arrangement, an IC-Pak Ion-Exclusion 7µm (7.8 x 300 mm) column and a mobile phase of 0.001N H2SO4 with isocratic elusion. The validation parameters show efficiency, adequate linearity, and standard deviation values (%RSD) from 0.018% and 4.650%. the quantification limits (LDC) were 50.76 mg/L for citric acid and 20.18 mg/L for oxalic acid and the detection limits (LDD) were 0.6 mg/L for both acids. The method was applied in the analysis of organic acids in fermentation broths of Aspergillus niger. 2020 artículo científico 0012-7353 https://www.redalyc.org/articulo.oa?id=49663642003 https://www.redalyc.org/journal/496/49663642003/ https://www.redalyc.org/journal/496/49663642003/html/ https://www.redalyc.org/journal/496/49663642003/49663642003.epub https://www.redalyc.org/journal/496/49663642003/movil 10.15446/dyna.v87n212.76240 en http://www.redalyc.org/revista.oa?id=496 Dyna application/pdf Universidad Nacional de Colombia Dyna (Colombia) Num.212 Vol.87 |
| title | Determination of citric and oxalic acid in fungi fermentation broth through HPLC-DAD and solid-phase extraction |
| topic | Ingeniería HPLC citric acid oxalic acid solid phase extraction fermentation with fungi |
| url | https://www.redalyc.org/articulo.oa?id=49663642003 https://www.redalyc.org/journal/496/49663642003/ https://www.redalyc.org/journal/496/49663642003/html/ https://www.redalyc.org/journal/496/49663642003/49663642003.epub https://www.redalyc.org/journal/496/49663642003/movil |