Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad Nacional de Colombia
2020
|
| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=49666177021 https://www.redalyc.org/journal/496/49666177021/ https://www.redalyc.org/journal/496/49666177021/html/ https://www.redalyc.org/journal/496/49666177021/49666177021.epub https://www.redalyc.org/journal/496/49666177021/movil |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866815533065699328 |
|---|---|
| author | Hugo Rueda-Rueda |
| author_facet | Hugo Rueda-Rueda |
| contents | Cutinases obtained from filamentous fungi: a comparison of screening methods Hugo Rueda-Rueda Erlide Prieto-Correa Carlos Jiménez-Junca Ingeniería cutinases flaxseed oil Filamentous fungi cutinase activity agroindustrial wastes Cutinases are secreted by filamentous fungi that and hydrolyze polymers. However, few selection methods for cutinases are available. Here, we studied three screening methods using 33 strains of filamentous fungi isolated from banana rachis with high potential to produce cutinases. In the first method, strains were grown in Czapec-Dox mineral medium containing flaxseed oil. We note that six strains of the genera Fusarium, Penicillium, and Mucor had cutinase activity. The second method evaluated strains with triacetin in rhodamine B, which indicated what strains had esterase property. Finally, strains were subjected to fermentation with flaxseed oil; lipolytic and cutinolytic activity were determined. The species identified as the best producers of cutinases were Fusarium fujikuroi and Penicillium chrysogenum, and we obtained two extracellular cutinases with activities of 33.5 U/mL and 39.4 U/mL respectively. Cutinase was confirmed via degradation of tomato cutin through FTIR. 2020 artículo científico 0012-7353 https://www.redalyc.org/articulo.oa?id=49666177021 https://www.redalyc.org/journal/496/49666177021/ https://www.redalyc.org/journal/496/49666177021/html/ https://www.redalyc.org/journal/496/49666177021/49666177021.epub https://www.redalyc.org/journal/496/49666177021/movil 10.15446/dyna.v87n214.83737 en http://www.redalyc.org/revista.oa?id=496 Dyna application/pdf Universidad Nacional de Colombia Dyna (Colombia) Num.214 Vol.87 |
| format | Artículo científico |
| id | redalyc_49666177021 |
| language | en |
| publishDate | 2020 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Cutinases obtained from filamentous fungi: a comparison of screening methods Hugo Rueda-Rueda Ingeniería cutinases flaxseed oil Filamentous fungi cutinase activity agroindustrial wastes Cutinases obtained from filamentous fungi: a comparison of screening methods Hugo Rueda-Rueda Erlide Prieto-Correa Carlos Jiménez-Junca Ingeniería cutinases flaxseed oil Filamentous fungi cutinase activity agroindustrial wastes Cutinases are secreted by filamentous fungi that and hydrolyze polymers. However, few selection methods for cutinases are available. Here, we studied three screening methods using 33 strains of filamentous fungi isolated from banana rachis with high potential to produce cutinases. In the first method, strains were grown in Czapec-Dox mineral medium containing flaxseed oil. We note that six strains of the genera Fusarium, Penicillium, and Mucor had cutinase activity. The second method evaluated strains with triacetin in rhodamine B, which indicated what strains had esterase property. Finally, strains were subjected to fermentation with flaxseed oil; lipolytic and cutinolytic activity were determined. The species identified as the best producers of cutinases were Fusarium fujikuroi and Penicillium chrysogenum, and we obtained two extracellular cutinases with activities of 33.5 U/mL and 39.4 U/mL respectively. Cutinase was confirmed via degradation of tomato cutin through FTIR. 2020 artículo científico 0012-7353 https://www.redalyc.org/articulo.oa?id=49666177021 https://www.redalyc.org/journal/496/49666177021/ https://www.redalyc.org/journal/496/49666177021/html/ https://www.redalyc.org/journal/496/49666177021/49666177021.epub https://www.redalyc.org/journal/496/49666177021/movil 10.15446/dyna.v87n214.83737 en http://www.redalyc.org/revista.oa?id=496 Dyna application/pdf Universidad Nacional de Colombia Dyna (Colombia) Num.214 Vol.87 |
| title | Cutinases obtained from filamentous fungi: a comparison of screening methods |
| topic | Ingeniería cutinases flaxseed oil Filamentous fungi cutinase activity agroindustrial wastes |
| url | https://www.redalyc.org/articulo.oa?id=49666177021 https://www.redalyc.org/journal/496/49666177021/ https://www.redalyc.org/journal/496/49666177021/html/ https://www.redalyc.org/journal/496/49666177021/49666177021.epub https://www.redalyc.org/journal/496/49666177021/movil |