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| Format: | Artículo científico |
| Language: | en |
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Universidad de Costa Rica
2016
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| Online Access: | https://www.redalyc.org/articulo.oa?id=499555370010 |
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Table of Contents:
- Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis : In Vitro Study Silvia Rodríguez-Chang Tatiana Ramírez-Mora Grettel Valle-Bourrouet Norman Rojas-Campos Daniel Chavarría-Bolaño Mauricio Montero-Aguilar Medicina faecalis Sodium hypochlorite Silver nanoparticles Antimicrobial efficacy The purpose of this study was to measure the antibacterial efficacy of a dispersion of silver nanoparticles (AgNP) in a citrate medium tested in two E. faecalis strains. AgNP were synthesized, and AgNP citrate medium (AgNP-CM) dispersion was prepared at a concentration of 100 μg/mL. The antibacterial efficacy of AgNP-CM dispersion was evaluated over two E. faecalis strains: ATCC29212 and a wild strain collected from human necrotic teeth. 5% sodium hypochlorite (NaOCl) and sterile saline solution were used as positive and negative controls. 5 and 30-minute contact tests were conducted and each experimental group were replicated 10 times. After 24 hours of incubation, the Log CFU/ mL were calculated. The AgNP obtained showed spherical shapes and had 30-60nm size. 5% NaOCl was able to completely eliminate both E. faecalis strains in all groups, showing a significant statistical difference when compared to AgNP-CM dispersion and negative control groups. AgNP-CM dispersion showed a statistically significant decrease in Log CFU/mL averages (p=0,0006) when compared to the sterile saline solution for the ATCC29212 strain during the 30-minute time. Between the 5-minute and 30-minute groups, a significant bacterial count decrease was also observed (p= 0,0128). The antibacterial efficacy of the dispersion was greater for the ATCC29212 strain than the wild strain, were the effect diminished. AgNP-CM dispersion showed a significantly lower antibacterial efficacy against E. faecalis than the 5% NaOCl at the tested times. 2016 artículo científico 1659-1046 https://www.redalyc.org/articulo.oa?id=499555370010 en http://www.redalyc.org/revista.oa?id=4995 Odovtos - International Journal of Dental Sciences application/pdf Universidad de Costa Rica Odovtos - International Journal of Dental Sciences (Costa Rica) Num.2 Vol.18