Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad de Costa Rica
2023
|
| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=499574842007 https://www.redalyc.org/journal/4995/499574842007/ https://www.redalyc.org/journal/4995/499574842007/html/ https://www.redalyc.org/journal/4995/499574842007/499574842007.epub https://www.redalyc.org/journal/4995/499574842007/movil |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866578900053655552 |
|---|---|
| author | Febe C. Vázquez-Vázquez |
| author_facet | Febe C. Vázquez-Vázquez |
| contents | Comparison of Two Obturation Techniques on Adhesion and Proliferation of Human Periodontal Ligament Fibroblasts Febe C. Vázquez-Vázquez Lourdes Y. Avalos-Piña Alejandra Rodríguez-Hidalgo Amaury Pozos-Guillén Patricia González-Alva Mauricio Montero-Aguilar Daniel Chavarría-Bolaños Marco A. Álvarez-Pérez Medicina AH Plus bioRoot RCS cytotoxicity biocompatibility The present study aimed to compare the adhesion and proliferation of human periodontal ligament fibroblasts (hPDL) in transverse sections of the teeth sealed with two different obturation techniques, BioRoot RCS/hydraulic obturation (HO) and AH-Plus/continuous-wave condensation (CWC). The techniques were tested using an in vitro model to simulate the interaction between periodontal tissues and the materials. The root canals were instrumented and sterilized. A total of 15 samples were obturated with BioRoot RCS/HO and 15 samples with AH-Plus/CWC. Then, roots were sectioned to obtain obturated teeth slices, and hPDL cells were seeded onto the root slices. The results were obtained at intervals of 4 and 24h for cell adhesion; and at 3,7,14, and 21 days for cell proliferation. Empty cell culture plates were use as controls. The cell adhesion was increased at 4 and 24h for both groups, with an increased response observed in the BioRoot RCS/HO group (p<0.05). The difference in cell proliferation was also found between experimental groups. After 14 days of culture, BioRoot RCS/HO group showed an increase response than control and AH-Plus/CWC groups (p<0.05), and after 21 days both groups behaved better than control group, with an increased response observed in the BioRoot RCS/HO group. This study demonstrated that both root canal sealers allow the attach and growth of periodontal ligament fibroblasts, with an increased biological response in the BioRoot RCS/HO group. 2023 artículo científico 1659-1046 https://www.redalyc.org/articulo.oa?id=499574842007 https://www.redalyc.org/journal/4995/499574842007/ https://www.redalyc.org/journal/4995/499574842007/html/ https://www.redalyc.org/journal/4995/499574842007/499574842007.epub https://www.redalyc.org/journal/4995/499574842007/movil 10.15517/IJDS.2022.52583 en http://www.redalyc.org/revista.oa?id=4995 Odovtos - International Journal of Dental Sciences application/pdf Universidad de Costa Rica Odovtos - International Journal of Dental Sciences (Costa Rica) Num.1 Vol.25 |
| format | Artículo científico |
| id | redalyc_499574842007 |
| language | en |
| publishDate | 2023 |
| publisher | Universidad de Costa Rica |
| spellingShingle | Comparison of Two Obturation Techniques on Adhesion and Proliferation of Human Periodontal Ligament Fibroblasts Febe C. Vázquez-Vázquez Medicina AH Plus bioRoot RCS cytotoxicity biocompatibility Comparison of Two Obturation Techniques on Adhesion and Proliferation of Human Periodontal Ligament Fibroblasts Febe C. Vázquez-Vázquez Lourdes Y. Avalos-Piña Alejandra Rodríguez-Hidalgo Amaury Pozos-Guillén Patricia González-Alva Mauricio Montero-Aguilar Daniel Chavarría-Bolaños Marco A. Álvarez-Pérez Medicina AH Plus bioRoot RCS cytotoxicity biocompatibility The present study aimed to compare the adhesion and proliferation of human periodontal ligament fibroblasts (hPDL) in transverse sections of the teeth sealed with two different obturation techniques, BioRoot RCS/hydraulic obturation (HO) and AH-Plus/continuous-wave condensation (CWC). The techniques were tested using an in vitro model to simulate the interaction between periodontal tissues and the materials. The root canals were instrumented and sterilized. A total of 15 samples were obturated with BioRoot RCS/HO and 15 samples with AH-Plus/CWC. Then, roots were sectioned to obtain obturated teeth slices, and hPDL cells were seeded onto the root slices. The results were obtained at intervals of 4 and 24h for cell adhesion; and at 3,7,14, and 21 days for cell proliferation. Empty cell culture plates were use as controls. The cell adhesion was increased at 4 and 24h for both groups, with an increased response observed in the BioRoot RCS/HO group (p<0.05). The difference in cell proliferation was also found between experimental groups. After 14 days of culture, BioRoot RCS/HO group showed an increase response than control and AH-Plus/CWC groups (p<0.05), and after 21 days both groups behaved better than control group, with an increased response observed in the BioRoot RCS/HO group. This study demonstrated that both root canal sealers allow the attach and growth of periodontal ligament fibroblasts, with an increased biological response in the BioRoot RCS/HO group. 2023 artículo científico 1659-1046 https://www.redalyc.org/articulo.oa?id=499574842007 https://www.redalyc.org/journal/4995/499574842007/ https://www.redalyc.org/journal/4995/499574842007/html/ https://www.redalyc.org/journal/4995/499574842007/499574842007.epub https://www.redalyc.org/journal/4995/499574842007/movil 10.15517/IJDS.2022.52583 en http://www.redalyc.org/revista.oa?id=4995 Odovtos - International Journal of Dental Sciences application/pdf Universidad de Costa Rica Odovtos - International Journal of Dental Sciences (Costa Rica) Num.1 Vol.25 |
| title | Comparison of Two Obturation Techniques on Adhesion and Proliferation of Human Periodontal Ligament Fibroblasts |
| topic | Medicina AH Plus bioRoot RCS cytotoxicity biocompatibility |
| url | https://www.redalyc.org/articulo.oa?id=499574842007 https://www.redalyc.org/journal/4995/499574842007/ https://www.redalyc.org/journal/4995/499574842007/html/ https://www.redalyc.org/journal/4995/499574842007/499574842007.epub https://www.redalyc.org/journal/4995/499574842007/movil |