Detección de anticuerpos anti-núcleo con inmunoensayo lineal. Correlación con inmunofluorescencia indirecta
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| Format: | Artículo científico |
| Language: | en |
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Federación Bioquímica de la Provincia de Buenos Aires
2005
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| _version_ | 1876479517951787008 |
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| author | Paula Londero |
| author_facet | Paula Londero |
| contents | Detección de anticuerpos anti-núcleo con inmunoensayo lineal. Correlación con inmunofluorescencia indirecta Paula Londero Nora Silvia Bovone María Cristina Fuente Mario Omar Eposto Biología immunoassay nonparametric statistics indirect immunofluorescence ANTI-NUCLEUS ANTIBODIES DETECTION BY LINE IMMUNOASSAY.CORRELATION WITH INDIRECT IMMUNOFLUORESCENCEThe most used screening test to detect autoantibodies in systemic diseases isindirect immunofluorescence (IIF) with Hep-2 cells. However, additional methods,such as double diffusion, immunoblotting, ELISA or line immunoassay(LIA), are used to identify specific antibodies profiles. The aim of this study was: a) To asses the probability of fine detection by a commercial LIA on serum samplesthat resulted positive by IIF screening and b) To asses the correlation between the patternsand titles observed. A hundred and sixty-one serum samples positive by IIF that hadalso been processed by LIA were selected. Hep-2 slides of Kallestad and commercial kitInnolia-ANA of Innogenetics were used. Sixty samples were also studied for anti-dsDNA byIIF with Crithidia Lucilliae slides of Kallestad. Sixty per cent of the samples with homogeneuspattern were positive by LIA, with a prevalence of anti-histones and anti-dsDNA. Thefinding of antibodies anti-ENA in this group was important. In the speckle sample, 73%were positive. In detecting anti-ENA, speckle sample was more forceful than homogeneouspattern which showed little information about a specific antibodies profile. Anticentromerepattern did not need confirmation. The probability of finding fine reactivity by LIA isincreased with the rise in title, but its search for low titles is still justified. 2005 artículo científico 0325-2957 https://www.redalyc.org/articulo.oa?id=53539402 en http://www.redalyc.org/revista.oa?id=535 Acta Bioquímica Clínica Latinoamericana application/pdf Federación Bioquímica de la Provincia de Buenos Aires Acta Bioquímica Clínica Latinoamericana (Argentina) Num.4 Vol.39 |
| format | Artículo científico |
| id | redalyc_53539402 |
| institution | Redalyc |
| language | en |
| publishDate | 2005 |
| publisher | Federación Bioquímica de la Provincia de Buenos Aires |
| spellingShingle | Detección de anticuerpos anti-núcleo con inmunoensayo lineal. Correlación con inmunofluorescencia indirecta Paula Londero Biología immunoassay nonparametric statistics indirect immunofluorescence Detección de anticuerpos anti-núcleo con inmunoensayo lineal. Correlación con inmunofluorescencia indirecta Paula Londero Nora Silvia Bovone María Cristina Fuente Mario Omar Eposto Biología immunoassay nonparametric statistics indirect immunofluorescence ANTI-NUCLEUS ANTIBODIES DETECTION BY LINE IMMUNOASSAY.CORRELATION WITH INDIRECT IMMUNOFLUORESCENCEThe most used screening test to detect autoantibodies in systemic diseases isindirect immunofluorescence (IIF) with Hep-2 cells. However, additional methods,such as double diffusion, immunoblotting, ELISA or line immunoassay(LIA), are used to identify specific antibodies profiles. The aim of this study was: a) To asses the probability of fine detection by a commercial LIA on serum samplesthat resulted positive by IIF screening and b) To asses the correlation between the patternsand titles observed. A hundred and sixty-one serum samples positive by IIF that hadalso been processed by LIA were selected. Hep-2 slides of Kallestad and commercial kitInnolia-ANA of Innogenetics were used. Sixty samples were also studied for anti-dsDNA byIIF with Crithidia Lucilliae slides of Kallestad. Sixty per cent of the samples with homogeneuspattern were positive by LIA, with a prevalence of anti-histones and anti-dsDNA. Thefinding of antibodies anti-ENA in this group was important. In the speckle sample, 73%were positive. In detecting anti-ENA, speckle sample was more forceful than homogeneouspattern which showed little information about a specific antibodies profile. Anticentromerepattern did not need confirmation. The probability of finding fine reactivity by LIA isincreased with the rise in title, but its search for low titles is still justified. 2005 artículo científico 0325-2957 https://www.redalyc.org/articulo.oa?id=53539402 en http://www.redalyc.org/revista.oa?id=535 Acta Bioquímica Clínica Latinoamericana application/pdf Federación Bioquímica de la Provincia de Buenos Aires Acta Bioquímica Clínica Latinoamericana (Argentina) Num.4 Vol.39 |
| title | Detección de anticuerpos anti-núcleo con inmunoensayo lineal. Correlación con inmunofluorescencia indirecta |
| topic | Biología immunoassay nonparametric statistics indirect immunofluorescence |
| url | https://www.redalyc.org/articulo.oa?id=53539402 |