Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells
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| Format: | Artículo científico |
| Language: | en |
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Universidad del Rosario
2011
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| _version_ | 1876481955747332096 |
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| author | Lilian Chuaire-Noack |
| author_facet | Lilian Chuaire-Noack |
| contents | Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells Lilian Chuaire-Noack Pedro Monterrey-Gutiérrez César E. Payán-Gómez Salud cell aging Gene expression replicative senescence GAPDH can bind single-strand telomere DNA both in vitro and in vivo. Thus, it was hypothesised that GAPDH has an important role in protecting the telomeres, role that could be shared with TRF2, a well-known telomeric protein involved in a myriad of functions related to telomere homeostasis. Objective: The aim of this study was to determine if there was a correlation between the expression of these genes in the in vitro ovarian surface epithelium. Materials and methods: The relative expression of each gene was established by qRT-PCR in primary cell cultures of the ovarian surface epithelium from 22 healthy mestizo Colombian donors. Results: The Kendall and Spearman non-parametric tests established a significant correlation between the levels of expression in subsequent passages of the cell line, in an age-independent way. Conclusion: Our findings suggest a synergistic effect between TRF2 and GAPDH that could counter telomere shortening in vitro. 2011 artículo científico 1692-7273 https://www.redalyc.org/articulo.oa?id=56222319002 en http://www.redalyc.org/revista.oa?id=562 Revista Ciencias de la Salud application/pdf Universidad del Rosario Revista Ciencias de la Salud (Colombia) Num.3 Vol.9 |
| format | Artículo científico |
| id | redalyc_56222319002 |
| institution | Redalyc |
| language | en |
| publishDate | 2011 |
| publisher | Universidad del Rosario |
| spellingShingle | Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells Lilian Chuaire-Noack Salud cell aging Gene expression replicative senescence Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells Lilian Chuaire-Noack Pedro Monterrey-Gutiérrez César E. Payán-Gómez Salud cell aging Gene expression replicative senescence GAPDH can bind single-strand telomere DNA both in vitro and in vivo. Thus, it was hypothesised that GAPDH has an important role in protecting the telomeres, role that could be shared with TRF2, a well-known telomeric protein involved in a myriad of functions related to telomere homeostasis. Objective: The aim of this study was to determine if there was a correlation between the expression of these genes in the in vitro ovarian surface epithelium. Materials and methods: The relative expression of each gene was established by qRT-PCR in primary cell cultures of the ovarian surface epithelium from 22 healthy mestizo Colombian donors. Results: The Kendall and Spearman non-parametric tests established a significant correlation between the levels of expression in subsequent passages of the cell line, in an age-independent way. Conclusion: Our findings suggest a synergistic effect between TRF2 and GAPDH that could counter telomere shortening in vitro. 2011 artículo científico 1692-7273 https://www.redalyc.org/articulo.oa?id=56222319002 en http://www.redalyc.org/revista.oa?id=562 Revista Ciencias de la Salud application/pdf Universidad del Rosario Revista Ciencias de la Salud (Colombia) Num.3 Vol.9 |
| title | Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells |
| topic | Salud cell aging Gene expression replicative senescence |
| url | https://www.redalyc.org/articulo.oa?id=56222319002 |