Immobilization of mannanase on magnetic chitosan microspheres
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| Format: | Artículo científico |
| Langue: | en |
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Sociedad Mexicana de Física A.C.
2012
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| _version_ | 1876434974777802752 |
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| author | L.V. Zuluaga |
| author_facet | L.V. Zuluaga |
| contents | Immobilization of mannanase on magnetic chitosan microspheres L.V. Zuluaga O.H Giraldo C.E. Orrego Física, Astronomía y Matemáticas entrapment cross linking immobilization Magnetic chitosan microspheres Chitosan has been widely investigated for the enzyme immobilization. In this study, magnetic chitosan microspheres (MCM) were synthesized with a ferrofluid, using ammonium bicarbonate to make insoluble chitosan; these microspheres were used for enzyme immobilization. Acetic acid (1%w/v) solution was used as solvent of chitosan dispersion (2%w/v), this dispersion was let react with a ferrofluid (2%v/v) for 2 hours with continuous stirring, then the enzyme was added, and after a while all solution was dried in a spray dryer obtaining magnetic chitosan microspheres with immobilized enzyme, characterization of microspheres were performed with SEM/ESEM micrographs confirmed spherical morphology. Energy-Dispersive X-Ray Spectroscopy (EDX) was used for the detection of iron in the samples. Additional the magnetization profile were obtained. Mannanase was immobilized on magnetic chitosan microspheres by entrapment and cross-linking with glutaraldehyde. The immobilization conditions were investigated achieving the conservation of catalytic activity. The catalytic behavior of the immobilized mannanase was acceptable as compared with the same characteristics of the free enzyme. The magnetic characteristic of these materials allows easy removal of enzyme after use. 2012 artículo científico 0035-001X https://www.redalyc.org/articulo.oa?id=57030392011 en http://www.redalyc.org/revista.oa?id=570 Revista Mexicana de Física application/pdf Sociedad Mexicana de Física A.C. Revista Mexicana de Física (México) Num.2 Vol.58 |
| format | Artículo científico |
| id | redalyc_57030392011 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Sociedad Mexicana de Física A.C. |
| spellingShingle | Immobilization of mannanase on magnetic chitosan microspheres L.V. Zuluaga Física, Astronomía y Matemáticas entrapment cross linking immobilization Magnetic chitosan microspheres Immobilization of mannanase on magnetic chitosan microspheres L.V. Zuluaga O.H Giraldo C.E. Orrego Física, Astronomía y Matemáticas entrapment cross linking immobilization Magnetic chitosan microspheres Chitosan has been widely investigated for the enzyme immobilization. In this study, magnetic chitosan microspheres (MCM) were synthesized with a ferrofluid, using ammonium bicarbonate to make insoluble chitosan; these microspheres were used for enzyme immobilization. Acetic acid (1%w/v) solution was used as solvent of chitosan dispersion (2%w/v), this dispersion was let react with a ferrofluid (2%v/v) for 2 hours with continuous stirring, then the enzyme was added, and after a while all solution was dried in a spray dryer obtaining magnetic chitosan microspheres with immobilized enzyme, characterization of microspheres were performed with SEM/ESEM micrographs confirmed spherical morphology. Energy-Dispersive X-Ray Spectroscopy (EDX) was used for the detection of iron in the samples. Additional the magnetization profile were obtained. Mannanase was immobilized on magnetic chitosan microspheres by entrapment and cross-linking with glutaraldehyde. The immobilization conditions were investigated achieving the conservation of catalytic activity. The catalytic behavior of the immobilized mannanase was acceptable as compared with the same characteristics of the free enzyme. The magnetic characteristic of these materials allows easy removal of enzyme after use. 2012 artículo científico 0035-001X https://www.redalyc.org/articulo.oa?id=57030392011 en http://www.redalyc.org/revista.oa?id=570 Revista Mexicana de Física application/pdf Sociedad Mexicana de Física A.C. Revista Mexicana de Física (México) Num.2 Vol.58 |
| title | Immobilization of mannanase on magnetic chitosan microspheres |
| topic | Física, Astronomía y Matemáticas entrapment cross linking immobilization Magnetic chitosan microspheres |
| url | https://www.redalyc.org/articulo.oa?id=57030392011 |