Factors affecting in vitro shoot regeneration in hypocotyls of brinjal (Solanum melongena L.) in the early steps of Agrobacterium-mediated transformation
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| Format: | Artículo científico |
| Language: | en |
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Society for Promotion of Horticulture
2015
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| _version_ | 1876486830485929984 |
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| author | D.P. Prakash |
| author_facet | D.P. Prakash |
| contents | Factors affecting in vitro shoot regeneration in hypocotyls of brinjal (Solanum melongena L.) in the early steps of Agrobacterium-mediated transformation D.P. Prakash Y.L. Ramachandra Vageeshbabu S. Hanur Agrociencias pre PCR Eggplant explant characters culture on high BAP An attempt was made to assess the effect of size, age and position of the explant, pre-culture and high cytokinin concentration in the pre-culture medium on shoot regeneration in brinjal hypocotyls co-cultivated with Agrobacterium. The study was carried out using hypocotyl explants of brinjal cv. Manjarigota, Agrobacterium strain A208 and shoot regeneration medium (full-strength basal MS medium, 2μM BAP + 0.05μM NAA, 3% sucrose and 0.8% agar) containing Cefotaxime (250-500mg l-1) and Kanamycin (100mg l-1). Hypocotyl explants showed callus initiation and shoot regeneration response after 10-12 and 20-22 days of culture, respectively. Five-day-old explants did not survive Agrobacterium infection, and ten-day-old explants showed higher shoot regeneration (29±1.91%) than older explants. Explants of medium size (1cm long; 32±2.62%) from the apical region (38.57±2.61%) showed better shootregeneration ability than explants of any other size or region. A period of four days of pre-culture (33.33±3.76) was optimal best for best shoot-regeneration in hypocotyl explants. No regeneration was seen in hypocotyl explants at shorter or longer pre-culture period. High cytokinin (10μM) in shoot regeneration medium during pre-culture enhanced shoot regeneration response (47.27±2.98%) in explants co-cultivated with Agrobacterium. Effects of various factors documented in this study will be useful in developing an efficient Agrobacterium-mediated transformation protocol in brinjal cv. Manjarigota. 2015 artículo científico 0973-354X https://www.redalyc.org/articulo.oa?id=577061452004 en http://www.redalyc.org/revista.oa?id=5770 Journal of Horticultural Sciences application/pdf Society for Promotion of Horticulture Journal of Horticultural Sciences (India) Num.2 Vol.10 |
| format | Artículo científico |
| id | redalyc_577061452004 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Society for Promotion of Horticulture |
| spellingShingle | Factors affecting in vitro shoot regeneration in hypocotyls of brinjal (Solanum melongena L.) in the early steps of Agrobacterium-mediated transformation D.P. Prakash Agrociencias pre PCR Eggplant explant characters culture on high BAP Factors affecting in vitro shoot regeneration in hypocotyls of brinjal (Solanum melongena L.) in the early steps of Agrobacterium-mediated transformation D.P. Prakash Y.L. Ramachandra Vageeshbabu S. Hanur Agrociencias pre PCR Eggplant explant characters culture on high BAP An attempt was made to assess the effect of size, age and position of the explant, pre-culture and high cytokinin concentration in the pre-culture medium on shoot regeneration in brinjal hypocotyls co-cultivated with Agrobacterium. The study was carried out using hypocotyl explants of brinjal cv. Manjarigota, Agrobacterium strain A208 and shoot regeneration medium (full-strength basal MS medium, 2μM BAP + 0.05μM NAA, 3% sucrose and 0.8% agar) containing Cefotaxime (250-500mg l-1) and Kanamycin (100mg l-1). Hypocotyl explants showed callus initiation and shoot regeneration response after 10-12 and 20-22 days of culture, respectively. Five-day-old explants did not survive Agrobacterium infection, and ten-day-old explants showed higher shoot regeneration (29±1.91%) than older explants. Explants of medium size (1cm long; 32±2.62%) from the apical region (38.57±2.61%) showed better shootregeneration ability than explants of any other size or region. A period of four days of pre-culture (33.33±3.76) was optimal best for best shoot-regeneration in hypocotyl explants. No regeneration was seen in hypocotyl explants at shorter or longer pre-culture period. High cytokinin (10μM) in shoot regeneration medium during pre-culture enhanced shoot regeneration response (47.27±2.98%) in explants co-cultivated with Agrobacterium. Effects of various factors documented in this study will be useful in developing an efficient Agrobacterium-mediated transformation protocol in brinjal cv. Manjarigota. 2015 artículo científico 0973-354X https://www.redalyc.org/articulo.oa?id=577061452004 en http://www.redalyc.org/revista.oa?id=5770 Journal of Horticultural Sciences application/pdf Society for Promotion of Horticulture Journal of Horticultural Sciences (India) Num.2 Vol.10 |
| title | Factors affecting in vitro shoot regeneration in hypocotyls of brinjal (Solanum melongena L.) in the early steps of Agrobacterium-mediated transformation |
| topic | Agrociencias pre PCR Eggplant explant characters culture on high BAP |
| url | https://www.redalyc.org/articulo.oa?id=577061452004 |