Detection and quantification of Alternaria solani in tomato by real time PCR
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Society for Promotion of Horticulture
2014
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| _version_ | 1876449177563561984 |
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| author | P. Chowdappa |
| author_facet | P. Chowdappa |
| contents | Detection and quantification of Alternaria solani in tomato by real time PCR P. Chowdappa B.J. Nirmal Kumar B. Reddi Bhargavi K.R. Hema S.P. Mohan Kumar Agrociencias early blight Real Time PCR Alternaria solani molecular diagnosis A conventional and real-time PCR assays using SYBR Green for the detection and quantification of A. solani have been developed and validated. A primer set (ALP and ITS4) designed from the ITS region of A. linicola/ A. solani complex, yielded a 536 bp product when DNA from 38 isolates of A. solani were amplified. No product was amplified from A. alternata, A. brassicae, A. brassicicola, A.helianthi, A. porri, A. sesami, A.carthami, A.ricini, Colletotrichum gloeosporioides, C. capsici, C. falcatum, Cercospora canescens, C. capsici, Phytophthora infestans, Sclerotium rolfsii, Fusarium equiseti, F. oxysporum, Rhizoctonia solani, Phoma exigua, Curvularia spp and Drechslera. In addition, ALP/ITS4 primers were successfully utilized in real-time PCR assays of A. solani. The efficiency of conventional and real-time PCR assays was compared. The conventional PCR was able to detect the pathogen on symptomatic artificially infected tomato plants 5 days after pathogen inoculation. The detection limit was 100 conidia and 10 pg of DNA in the case of conventional PCR. Real-time PCR exhibited a detection limit 10 times lower (10 conidia, 10fg of DNA). The application of real time PCR assay for rapid detection of A.solani in infected tomato plant material is discussed. 2014 artículo científico 0973-354X https://www.redalyc.org/articulo.oa?id=577061808014 en http://www.redalyc.org/revista.oa?id=5770 Journal of Horticultural Sciences application/pdf Society for Promotion of Horticulture Journal of Horticultural Sciences (India) Num.2 Vol.9 |
| format | Artículo científico |
| id | redalyc_577061808014 |
| institution | Redalyc |
| language | en |
| publishDate | 2014 |
| publisher | Society for Promotion of Horticulture |
| spellingShingle | Detection and quantification of Alternaria solani in tomato by real time PCR P. Chowdappa Agrociencias early blight Real Time PCR Alternaria solani molecular diagnosis Detection and quantification of Alternaria solani in tomato by real time PCR P. Chowdappa B.J. Nirmal Kumar B. Reddi Bhargavi K.R. Hema S.P. Mohan Kumar Agrociencias early blight Real Time PCR Alternaria solani molecular diagnosis A conventional and real-time PCR assays using SYBR Green for the detection and quantification of A. solani have been developed and validated. A primer set (ALP and ITS4) designed from the ITS region of A. linicola/ A. solani complex, yielded a 536 bp product when DNA from 38 isolates of A. solani were amplified. No product was amplified from A. alternata, A. brassicae, A. brassicicola, A.helianthi, A. porri, A. sesami, A.carthami, A.ricini, Colletotrichum gloeosporioides, C. capsici, C. falcatum, Cercospora canescens, C. capsici, Phytophthora infestans, Sclerotium rolfsii, Fusarium equiseti, F. oxysporum, Rhizoctonia solani, Phoma exigua, Curvularia spp and Drechslera. In addition, ALP/ITS4 primers were successfully utilized in real-time PCR assays of A. solani. The efficiency of conventional and real-time PCR assays was compared. The conventional PCR was able to detect the pathogen on symptomatic artificially infected tomato plants 5 days after pathogen inoculation. The detection limit was 100 conidia and 10 pg of DNA in the case of conventional PCR. Real-time PCR exhibited a detection limit 10 times lower (10 conidia, 10fg of DNA). The application of real time PCR assay for rapid detection of A.solani in infected tomato plant material is discussed. 2014 artículo científico 0973-354X https://www.redalyc.org/articulo.oa?id=577061808014 en http://www.redalyc.org/revista.oa?id=5770 Journal of Horticultural Sciences application/pdf Society for Promotion of Horticulture Journal of Horticultural Sciences (India) Num.2 Vol.9 |
| title | Detection and quantification of Alternaria solani in tomato by real time PCR |
| topic | Agrociencias early blight Real Time PCR Alternaria solani molecular diagnosis |
| url | https://www.redalyc.org/articulo.oa?id=577061808014 |