Low cost strategy for micropropagation of Lilium Asiatic hybrid cv. Toscana

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Autor principal: R. Kaur
Formato: Artículo científico
Lenguaje:en
Publicado: Society for Promotion of Horticulture 2006
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author R. Kaur
author_facet R. Kaur
contents Low cost strategy for micropropagation of Lilium Asiatic hybrid cv. Toscana R. Kaur Neetu Thakur D. R. Sharma Agrociencias low Lilium cost strategy micropropagation A low cost protocol for in vitro propagation of Lilium cv. Toscana has been developed through incorporation of cost-effective media components. MS medium supplemented with 0.75 mg I* BAP (6-benzylaminopurine) and 0.5 mg 1' NAA (a-naphthalene-acetic acid) was prepared with tapioca granules, table sugar and tap water in different combinations in place of agar-agar, sucrose and distilled water, respectively. Culture medium containing all the cost effective components was found to be the best for in vitro establishment of cultures yielding 6.00 bulblets per explant and medium supplemented with tapioca granules as cost effective component was found to be the best for in vitro multiplication of bulblets giving 3.70 bulblets per in vitro formed bulblet five weeks from third subculture. Tapioca supplemented MS medium containing 1 mg I' NAA was significantly better than all the other modified media giving 86.62% in vitro rooting, 2.86 average root number and 4.60 cm average root length. For hardening of in vitro rooted bulblets, coco peat, peat moss and coco peat in combination with peat moss were found to be at par giving 100% survival. 2006 artículo científico 0973-354X https://www.redalyc.org/articulo.oa?id=577063420004 en http://www.redalyc.org/revista.oa?id=5770 Journal of Horticultural Sciences application/pdf Society for Promotion of Horticulture Journal of Horticultural Sciences (India) Num.1 Vol.1
format Artículo científico
id redalyc_577063420004
institution Redalyc
language en
publishDate 2006
publisher Society for Promotion of Horticulture
spellingShingle Low cost strategy for micropropagation of Lilium Asiatic hybrid cv. Toscana
R. Kaur
Agrociencias
low
Lilium
cost strategy
micropropagation
Low cost strategy for micropropagation of Lilium Asiatic hybrid cv. Toscana R. Kaur Neetu Thakur D. R. Sharma Agrociencias low Lilium cost strategy micropropagation A low cost protocol for in vitro propagation of Lilium cv. Toscana has been developed through incorporation of cost-effective media components. MS medium supplemented with 0.75 mg I* BAP (6-benzylaminopurine) and 0.5 mg 1' NAA (a-naphthalene-acetic acid) was prepared with tapioca granules, table sugar and tap water in different combinations in place of agar-agar, sucrose and distilled water, respectively. Culture medium containing all the cost effective components was found to be the best for in vitro establishment of cultures yielding 6.00 bulblets per explant and medium supplemented with tapioca granules as cost effective component was found to be the best for in vitro multiplication of bulblets giving 3.70 bulblets per in vitro formed bulblet five weeks from third subculture. Tapioca supplemented MS medium containing 1 mg I' NAA was significantly better than all the other modified media giving 86.62% in vitro rooting, 2.86 average root number and 4.60 cm average root length. For hardening of in vitro rooted bulblets, coco peat, peat moss and coco peat in combination with peat moss were found to be at par giving 100% survival. 2006 artículo científico 0973-354X https://www.redalyc.org/articulo.oa?id=577063420004 en http://www.redalyc.org/revista.oa?id=5770 Journal of Horticultural Sciences application/pdf Society for Promotion of Horticulture Journal of Horticultural Sciences (India) Num.1 Vol.1
title Low cost strategy for micropropagation of Lilium Asiatic hybrid cv. Toscana
topic Agrociencias
low
Lilium
cost strategy
micropropagation
url https://www.redalyc.org/articulo.oa?id=577063420004