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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Society for Promotion of Horticulture
2021
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| Accesso online: | https://www.redalyc.org/articulo.oa?id=577074023014 https://www.redalyc.org/journal/5770/577074023014/ https://www.redalyc.org/journal/5770/577074023014/html/ https://www.redalyc.org/journal/5770/577074023014/577074023014.epub https://www.redalyc.org/journal/5770/577074023014/movil https://doi.org/10.24154/JHS.2021.v16i01.014 |
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Sommario:
- Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification K.V Ravishankar R Vasudeva B Hemanth P Nischita B.R Sthapit V.A Parthasarathy V.R Rao Agrociencias Cross generation sequencing (NGS) Microsatellite markers and Next species amplification Garcinia indica Garcinia indica popularly known as ‘Kokum’ or Murugalu”, is a medium sized evergreen tree found in western-ghats of India. This tree species is highly exploited to produce anti-obesity drugs and culinary purposes. Its population is threatened by over exploitation and loss of habitat. Development of microsatellite markers would help in understanding genetic structure and further to develop appropriate conservation strategies. In this study, using next generation sequencing platform Illumina Hiseq 2000, we have sequenced partial genome of G. indica and identified 3725 microsatellites. Forty-eight microsatellite markers were analyzed using 30 accessions. Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922. Allele per locus ranged from 3 to 33 per locus. Probability of identity values ranged from 0.00329 to 0.30489. Cross species amplification SSR primers in the related species, showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta). 2021 otro 0973-354X https://www.redalyc.org/articulo.oa?id=577074023014 https://www.redalyc.org/journal/5770/577074023014/ https://www.redalyc.org/journal/5770/577074023014/html/ https://www.redalyc.org/journal/5770/577074023014/577074023014.epub https://www.redalyc.org/journal/5770/577074023014/movil https://doi.org/10.24154/JHS.2021.v16i01.014 en http://www.redalyc.org/revista.oa?id=5770 Journal of Horticultural Sciences application/pdf Society for Promotion of Horticulture Journal of Horticultural Sciences (India) Num.1 Vol.16