Procesamiento postraduccional de la porción no opioide de la proencefalina (Synencefalina) en el cerebro de la rata
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Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
2000
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| _version_ | 1876425401226493952 |
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| author | Miguel Asai |
| author_facet | Miguel Asai |
| contents | Procesamiento postraduccional de la porción no opioide de la proencefalina (Synencefalina) en el cerebro de la rata Miguel Asai Gabriel Linares Pablo Agustín Gilberto Matamoros Trejo Medicina post release Striatum synenkephalin translational processing Several protein hormones and neuropeptides are derived byenzymatic cleavage from a large and inactive peptide precursor.The enkephalins precursor, the proenkephalin A (PA) hasbeen deduced by sequencing cloned DNA. The PA containsfour copies of Met-Enkephalin and one copy each of Leu-Enkephalin,heptapeptide and octapeptide. Proenkephalin (aminoacids 1-70)does not contain any opioid peptide sequence andhas been named synenkephalin (Syn). Initial evidences suggestedthat Syn is produced and secreted as an intact moleculeor as part of the precursors in the adult brain and adrenalmedulla, respectively. However, under specific physiologicalconditions, i.e., proliferating immune cells, Syn (8.6 kDa) itis processed to low molecular weight peptides (1.0 kDa). Untilnow there is no evidence of the production or release of a 1.0kDa peptide derived from synenkephalin in the adult rat brain.In this work we studied the postranslational processing ofsynenkephalin in rat striatum, either in whole tissue or in vitrorelease conditions.Male Wistar rats were used throughout this experiment. Therats were sacrificad by decapitation and the striatum removed.Samples were divided into three groups: 1) Thes striatum (n=8)was homogenized and centrifuged; the supernatant was lyophilizedand resuspended in 2 ml water. 2) Striatum was dissectedand sliced in two directions at 90° at 300 mm intervalswith a tissue chopper. The slices were used for the assessmentof the in vitro release of synenkephalin and Met-Enkephalin,using potassium as depolarized stimulus. 3) The synaptosomalfraction was obtained from striatum; samples were depolarizedwith potassium 55 mM, and the perfusates were lyophilizedand resuspended in 2 ml water. Samples were individuallyapplied to a Sephadex G-50 column... 2000 artículo científico 0185-3325 https://www.redalyc.org/articulo.oa?id=58232302 en http://www.redalyc.org/revista.oa?id=582 Salud Mental application/pdf Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz Salud Mental (México) Num.3 Vol.23 |
| format | Artículo científico |
| id | redalyc_58232302 |
| institution | Redalyc |
| language | en |
| publishDate | 2000 |
| publisher | Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz |
| spellingShingle | Procesamiento postraduccional de la porción no opioide de la proencefalina (Synencefalina) en el cerebro de la rata Miguel Asai Medicina post release Striatum synenkephalin translational processing Procesamiento postraduccional de la porción no opioide de la proencefalina (Synencefalina) en el cerebro de la rata Miguel Asai Gabriel Linares Pablo Agustín Gilberto Matamoros Trejo Medicina post release Striatum synenkephalin translational processing Several protein hormones and neuropeptides are derived byenzymatic cleavage from a large and inactive peptide precursor.The enkephalins precursor, the proenkephalin A (PA) hasbeen deduced by sequencing cloned DNA. The PA containsfour copies of Met-Enkephalin and one copy each of Leu-Enkephalin,heptapeptide and octapeptide. Proenkephalin (aminoacids 1-70)does not contain any opioid peptide sequence andhas been named synenkephalin (Syn). Initial evidences suggestedthat Syn is produced and secreted as an intact moleculeor as part of the precursors in the adult brain and adrenalmedulla, respectively. However, under specific physiologicalconditions, i.e., proliferating immune cells, Syn (8.6 kDa) itis processed to low molecular weight peptides (1.0 kDa). Untilnow there is no evidence of the production or release of a 1.0kDa peptide derived from synenkephalin in the adult rat brain.In this work we studied the postranslational processing ofsynenkephalin in rat striatum, either in whole tissue or in vitrorelease conditions.Male Wistar rats were used throughout this experiment. Therats were sacrificad by decapitation and the striatum removed.Samples were divided into three groups: 1) Thes striatum (n=8)was homogenized and centrifuged; the supernatant was lyophilizedand resuspended in 2 ml water. 2) Striatum was dissectedand sliced in two directions at 90° at 300 mm intervalswith a tissue chopper. The slices were used for the assessmentof the in vitro release of synenkephalin and Met-Enkephalin,using potassium as depolarized stimulus. 3) The synaptosomalfraction was obtained from striatum; samples were depolarizedwith potassium 55 mM, and the perfusates were lyophilizedand resuspended in 2 ml water. Samples were individuallyapplied to a Sephadex G-50 column... 2000 artículo científico 0185-3325 https://www.redalyc.org/articulo.oa?id=58232302 en http://www.redalyc.org/revista.oa?id=582 Salud Mental application/pdf Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz Salud Mental (México) Num.3 Vol.23 |
| title | Procesamiento postraduccional de la porción no opioide de la proencefalina (Synencefalina) en el cerebro de la rata |
| topic | Medicina post release Striatum synenkephalin translational processing |
| url | https://www.redalyc.org/articulo.oa?id=58232302 |