Comparación del análisis inmunoenzimático y la cromatografía de líquidos de alta eficiencia (EMIT y HPLC) para la medición de carbamacepina en muestras séricas
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz
2001
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| _version_ | 1876460183208591360 |
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| author | Julia Moreno |
| author_facet | Julia Moreno |
| contents | Comparación del análisis inmunoenzimático y la cromatografía de líquidos de alta eficiencia (EMIT y HPLC) para la medición de carbamacepina en muestras séricas Julia Moreno Orlando Jaimes Ana Ma. Dorantes Estela Nieves Guadalupe López Aurora Belmont Gerardo Heinze Medicina 10 11 HPLC EMIT epoxide The sera of 40 patients treated with carbamazepine were analyzedby enzyme immunoassay (EMIT) and high-performance liquidchromatography (HPLC) and the results were compared. Thechromatographic method includes carbamazepine extraction withmethylene chloride and its chromatographic separation in a NovaPak C18 column by using a mixture of 25% v/v acetonitrile inH2O pH 5.6. Detection was made in an absorbance detector at215 nm.Correlation between these two methods was 0.93. Confidencebands for predicting HPLC according to the EMIT with Working-Hotelling criterium showed 1.1, 0.5 and 1.6 µg/ml maximunerror at low, medium and high ranges, respectively with a p=0.05probability.The equation expressing the behavior of carbamazepinemeasurement by HPLC in function of EMIT is as follows:HPLC=0.824 x EMIT + 0.777Afterwards, the chromatographic method was used fordetermining the serum predosis levels of carbamacepine and 10,11-epoxi-carbamacepine in five epileptic patients that receivedan oral dosis of 200 mg of carbamacepine every 8 hrs as the onlyanticonvulsivant for controlling their crises. 2001 artículo científico 0185-3325 https://www.redalyc.org/articulo.oa?id=58252403 en http://www.redalyc.org/revista.oa?id=582 Salud Mental application/pdf Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz Salud Mental (México) Num.5 Vol.24 |
| format | Artículo científico |
| id | redalyc_58252403 |
| institution | Redalyc |
| language | en |
| publishDate | 2001 |
| publisher | Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz |
| spellingShingle | Comparación del análisis inmunoenzimático y la cromatografía de líquidos de alta eficiencia (EMIT y HPLC) para la medición de carbamacepina en muestras séricas Julia Moreno Medicina 10 11 HPLC EMIT epoxide Comparación del análisis inmunoenzimático y la cromatografía de líquidos de alta eficiencia (EMIT y HPLC) para la medición de carbamacepina en muestras séricas Julia Moreno Orlando Jaimes Ana Ma. Dorantes Estela Nieves Guadalupe López Aurora Belmont Gerardo Heinze Medicina 10 11 HPLC EMIT epoxide The sera of 40 patients treated with carbamazepine were analyzedby enzyme immunoassay (EMIT) and high-performance liquidchromatography (HPLC) and the results were compared. Thechromatographic method includes carbamazepine extraction withmethylene chloride and its chromatographic separation in a NovaPak C18 column by using a mixture of 25% v/v acetonitrile inH2O pH 5.6. Detection was made in an absorbance detector at215 nm.Correlation between these two methods was 0.93. Confidencebands for predicting HPLC according to the EMIT with Working-Hotelling criterium showed 1.1, 0.5 and 1.6 µg/ml maximunerror at low, medium and high ranges, respectively with a p=0.05probability.The equation expressing the behavior of carbamazepinemeasurement by HPLC in function of EMIT is as follows:HPLC=0.824 x EMIT + 0.777Afterwards, the chromatographic method was used fordetermining the serum predosis levels of carbamacepine and 10,11-epoxi-carbamacepine in five epileptic patients that receivedan oral dosis of 200 mg of carbamacepine every 8 hrs as the onlyanticonvulsivant for controlling their crises. 2001 artículo científico 0185-3325 https://www.redalyc.org/articulo.oa?id=58252403 en http://www.redalyc.org/revista.oa?id=582 Salud Mental application/pdf Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz Salud Mental (México) Num.5 Vol.24 |
| title | Comparación del análisis inmunoenzimático y la cromatografía de líquidos de alta eficiencia (EMIT y HPLC) para la medición de carbamacepina en muestras séricas |
| topic | Medicina 10 11 HPLC EMIT epoxide |
| url | https://www.redalyc.org/articulo.oa?id=58252403 |