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| Formato: | Artículo científico |
| Lenguaje: | es |
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Politécnico Colombiano Jaime Isaza Cadavid
2007
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| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=607866447010 |
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- Portable instruments based in multiband fluorescence transducers for biosensing applications G. PEZZOTTI A. TIBUZZI T. LAVECCHIA G. REA A. SERAFINI M.T. GIARDI Multidisciplinaria (Ciencias Naturales y Exactas) Sensor biosensor biomediator fluorimeter fluorescence Two prototypes of the miniaturized biosensor-based optical instrument has been designed and fabricated for fluorescence measurements of several biomediators in series or only one, with applications in environmental monitoring and agrofood analysis. It is a multicell system, every cell is made up by two modular sections: the bottom compartment with optical LED light excitations and a photodiode detector for fluorescence emission capture, and the top biocompatible compartment where the biosample is deposited. The prototypes has been implemented with genetically modified algae that were employed in the instrument experimental testing by monitoring pesticide pollution in water. Pesticides modify the photosystem II (PSII) activity in terms of fluorescence quenching. Results from measurements employing several C251 mutants and three different pesticides at increasing concentrations and incubation times are presented and discussed. 2007 artículo científico 1900-2351 https://www.redalyc.org/articulo.oa?id=607866447010 es http://www.redalyc.org/revista.oa?id=6078 Revista Politécnica application/pdf Politécnico Colombiano Jaime Isaza Cadavid Revista Politécnica (Colombia) Num.5 Vol.3