PHOTOMETRIC DETECTION OF ANTIBIOTIC RESIDUES IN MILK BY A MICROBIOLOGICAL SYSTEM

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Autor principal: O.G. NAGEL
Formato: Artículo científico
Lenguaje:en
Publicado: Universidad Nacional del Litoral 2011
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author O.G. NAGEL
author_facet O.G. NAGEL
contents PHOTOMETRIC DETECTION OF ANTIBIOTIC RESIDUES IN MILK BY A MICROBIOLOGICAL SYSTEM O.G. NAGEL R. L. ALTHUS M.L. GASPAROTTI M. P. MOLINA Veterinaria milk Antimicrobial detection capability photometric detection microbial inhibitor test The aim of this study was to determine the detection capabilities of the ResScreen microbiological system (BT and BS Bioassays) for 26 antimicrobial agents in milk by photometric measurement. In order to carry out this study 16 replicates of twelve concentrations using samples of milk from individual cows were tested. The detection capabilities, determined by means of logistic regression models for the BT and BS bioassays were, respectively (ug/l): amoxicillin (8, 5), ampicillin (8, 4), cloxacillin (42, 42), oxacillin (19, 17), penicillin G (4, 4), cefadroxil (120, 180), cephalexin (96, 150), cefoperazone (80, 120), ceftiofur (75, 90), cefuroxime (113, 140), clortetracycline (400, 3300), oxytetracycline (118, 630), tetracycline (180, 620), sulfadiazine (41200, 106), sulfamethazine (3300, 355), sulfamethoxazol (9800, 78), sulfathiazole (10700, 115), gentamycin (640), neomycin (1200), streptomycin (2600), erythromycin (350), lincomicin (350), tylosin (100), ciprofloxacin (2855), enrofloxacin (2000) and marbofloxacin (3900). The simultaneous use of both bioassays identifies betalactam, tetracycline and sulfamide residues in milk. Also, neomycin, tylosin and lincomycin residues can be detected, but their residues can be confused with betalactam antibiotics since these molecules produce positive results in BT and BS bioassays. 2011 artículo científico 1666-938X https://www.redalyc.org/articulo.oa?id=617776636005 en http://www.redalyc.org/revista.oa?id=6177 FAVE Sección Ciencias Veterinarias application/pdf Universidad Nacional del Litoral FAVE Sección Ciencias Veterinarias (Argentina) Num.2 Vol.10
format Artículo científico
id redalyc_617776636005
institution Redalyc
language en
publishDate 2011
publisher Universidad Nacional del Litoral
spellingShingle PHOTOMETRIC DETECTION OF ANTIBIOTIC RESIDUES IN MILK BY A MICROBIOLOGICAL SYSTEM
O.G. NAGEL
Veterinaria
milk
Antimicrobial
detection capability
photometric detection
microbial inhibitor test
PHOTOMETRIC DETECTION OF ANTIBIOTIC RESIDUES IN MILK BY A MICROBIOLOGICAL SYSTEM O.G. NAGEL R. L. ALTHUS M.L. GASPAROTTI M. P. MOLINA Veterinaria milk Antimicrobial detection capability photometric detection microbial inhibitor test The aim of this study was to determine the detection capabilities of the ResScreen microbiological system (BT and BS Bioassays) for 26 antimicrobial agents in milk by photometric measurement. In order to carry out this study 16 replicates of twelve concentrations using samples of milk from individual cows were tested. The detection capabilities, determined by means of logistic regression models for the BT and BS bioassays were, respectively (ug/l): amoxicillin (8, 5), ampicillin (8, 4), cloxacillin (42, 42), oxacillin (19, 17), penicillin G (4, 4), cefadroxil (120, 180), cephalexin (96, 150), cefoperazone (80, 120), ceftiofur (75, 90), cefuroxime (113, 140), clortetracycline (400, 3300), oxytetracycline (118, 630), tetracycline (180, 620), sulfadiazine (41200, 106), sulfamethazine (3300, 355), sulfamethoxazol (9800, 78), sulfathiazole (10700, 115), gentamycin (640), neomycin (1200), streptomycin (2600), erythromycin (350), lincomicin (350), tylosin (100), ciprofloxacin (2855), enrofloxacin (2000) and marbofloxacin (3900). The simultaneous use of both bioassays identifies betalactam, tetracycline and sulfamide residues in milk. Also, neomycin, tylosin and lincomycin residues can be detected, but their residues can be confused with betalactam antibiotics since these molecules produce positive results in BT and BS bioassays. 2011 artículo científico 1666-938X https://www.redalyc.org/articulo.oa?id=617776636005 en http://www.redalyc.org/revista.oa?id=6177 FAVE Sección Ciencias Veterinarias application/pdf Universidad Nacional del Litoral FAVE Sección Ciencias Veterinarias (Argentina) Num.2 Vol.10
title PHOTOMETRIC DETECTION OF ANTIBIOTIC RESIDUES IN MILK BY A MICROBIOLOGICAL SYSTEM
topic Veterinaria
milk
Antimicrobial
detection capability
photometric detection
microbial inhibitor test
url https://www.redalyc.org/articulo.oa?id=617776636005