Study of Carbohydrate Hydrolysis From Arracacha Roots (Arracacia Xanthorriza Bancroft) to Produce Fermentable Sugars
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| Format: | Artículo científico |
| Language: | en |
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Universidad Nacional de Colombia
2021
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| author | Darwin Carranza-Saavedra |
| author_facet | Darwin Carranza-Saavedra |
| contents | Study of Carbohydrate Hydrolysis From Arracacha Roots (Arracacia Xanthorriza Bancroft) to Produce Fermentable Sugars Darwin Carranza-Saavedra Jorge A. Alvarado-Nuñez José F. Solanilla-Duque Claudia P. Valenzuela-Real Ingeniería reducing sugars Chemical hydrolysis enzymatic hydrolysis fermentation inhibitors In Colombia, approximately 855 840 tons of arracacha are produced each year. The unsalable post-harvest arracacha root (Arracacia xanthorriza Bancroft) is not commercialized, mainly due to mechanical damage or small and misshapen roots. In this work, dry samples were characterized and subjected to two treatments: one using thermal hydrolysis, applying saturated steam at pressures of 0,1034 MPa, 0,2068 MPa, and 0,4137 MPa; and another one using hydrolysis with sulfuric acid in concentrations between 0,25-2,00 M. Then, the cake resulting from the hydrolysis and filtration process was enzymatically hydrolyzed (Liquozyme SC DS, Novozymes) at 1,5, 5 and 10 KNU/g (pH 6, 80 °C, 2 h). Fermentation inhibitors (acetic acid and furfural) were evaluated in the best pretreatment. The results showed that the treatment with sulfuric acid at 1,00 M (2 h) has high yields in reducing sugars added to enzymatic hydrolysis. The maximum level of fermentable carbohydrates per gram of dry sample (1,04 g/g) was also reached. Regarding the fermentation inhibitors of the reducing sugar, a higher concentration of acetic acid was found with a lower furfural content. Therefore, arracacha discards are a promising raw material to increase the supply of bioethanol. 2021 artículo científico 0120-5609 https://www.redalyc.org/articulo.oa?id=64379873006 https://www.redalyc.org/journal/643/64379873006/ https://www.redalyc.org/journal/643/64379873006/html/ https://www.redalyc.org/journal/643/64379873006/64379873006.epub https://www.redalyc.org/journal/643/64379873006/movil 10.15446/ing.investig.v41n2.87365 en http://www.redalyc.org/revista.oa?id=643 Ingeniería e Investigación application/pdf Universidad Nacional de Colombia Ingeniería e Investigación (Colombia) Num.2 Vol.41 |
| format | Artículo científico |
| id | redalyc_64379873006 |
| institution | Redalyc |
| language | en |
| publishDate | 2021 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Study of Carbohydrate Hydrolysis From Arracacha Roots (Arracacia Xanthorriza Bancroft) to Produce Fermentable Sugars Darwin Carranza-Saavedra Ingeniería reducing sugars Chemical hydrolysis enzymatic hydrolysis fermentation inhibitors Study of Carbohydrate Hydrolysis From Arracacha Roots (Arracacia Xanthorriza Bancroft) to Produce Fermentable Sugars Darwin Carranza-Saavedra Jorge A. Alvarado-Nuñez José F. Solanilla-Duque Claudia P. Valenzuela-Real Ingeniería reducing sugars Chemical hydrolysis enzymatic hydrolysis fermentation inhibitors In Colombia, approximately 855 840 tons of arracacha are produced each year. The unsalable post-harvest arracacha root (Arracacia xanthorriza Bancroft) is not commercialized, mainly due to mechanical damage or small and misshapen roots. In this work, dry samples were characterized and subjected to two treatments: one using thermal hydrolysis, applying saturated steam at pressures of 0,1034 MPa, 0,2068 MPa, and 0,4137 MPa; and another one using hydrolysis with sulfuric acid in concentrations between 0,25-2,00 M. Then, the cake resulting from the hydrolysis and filtration process was enzymatically hydrolyzed (Liquozyme SC DS, Novozymes) at 1,5, 5 and 10 KNU/g (pH 6, 80 °C, 2 h). Fermentation inhibitors (acetic acid and furfural) were evaluated in the best pretreatment. The results showed that the treatment with sulfuric acid at 1,00 M (2 h) has high yields in reducing sugars added to enzymatic hydrolysis. The maximum level of fermentable carbohydrates per gram of dry sample (1,04 g/g) was also reached. Regarding the fermentation inhibitors of the reducing sugar, a higher concentration of acetic acid was found with a lower furfural content. Therefore, arracacha discards are a promising raw material to increase the supply of bioethanol. 2021 artículo científico 0120-5609 https://www.redalyc.org/articulo.oa?id=64379873006 https://www.redalyc.org/journal/643/64379873006/ https://www.redalyc.org/journal/643/64379873006/html/ https://www.redalyc.org/journal/643/64379873006/64379873006.epub https://www.redalyc.org/journal/643/64379873006/movil 10.15446/ing.investig.v41n2.87365 en http://www.redalyc.org/revista.oa?id=643 Ingeniería e Investigación application/pdf Universidad Nacional de Colombia Ingeniería e Investigación (Colombia) Num.2 Vol.41 |
| title | Study of Carbohydrate Hydrolysis From Arracacha Roots (Arracacia Xanthorriza Bancroft) to Produce Fermentable Sugars |
| topic | Ingeniería reducing sugars Chemical hydrolysis enzymatic hydrolysis fermentation inhibitors |
| url | https://www.redalyc.org/articulo.oa?id=64379873006 https://www.redalyc.org/journal/643/64379873006/ https://www.redalyc.org/journal/643/64379873006/html/ https://www.redalyc.org/journal/643/64379873006/64379873006.epub https://www.redalyc.org/journal/643/64379873006/movil |