Selection of a culture medium for the growth of Pichia guilliermondii LEVICA- 27 as activator additive of ruminal fermentation
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| Format: | Artículo científico |
| Language: | en |
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Instituto de Ciencia Animal
2022
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| _version_ | 1876479107290628096 |
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| author | Bexy González Mora |
| author_facet | Bexy González Mora |
| contents | Selection of a culture medium for the growth of Pichia guilliermondii LEVICA- 27 as activator additive of ruminal fermentation Bexy González Mora Dailyn Sosa Cossio Yoandra Marrero Rodríguez Yaneisy García Hernández Nereyda Albelo Dorta Estudios Agrarios yeast biomass Molasses fermentation In order to select a non-conventional culture medium for the growth of Pichia guilliermondii LEVICA-27 as an activator additive for ruminal fermentation, two trials were carried out in which YPG medium was used (yeast extract 10 g/L, peptone 10 g/L and glucose 20 g/L) as control medium. In the first, seven culture media were evaluated for yeast growth at 24 hours of fermentation. While in the second, growth kinetics was performed in the selected medium of the first test. In addition, in the latter, the maximum specific growth rate, the biomass doubling time, and the stoichiometric balance of the selected medium were determined. At 24 hours of fermentation, there were not differences between the media studied and the YPG medium (p=0.073). Therefore, the latter can be replaced without affecting the strain growth in 24 h. In the kinetic study, there was a higher microbial concentration at 12 h (P<0.0001) in the medium containing molasses and urea. The maximum specific growth rate was lower (P=0.0260) and the biomass doubling time was higher (P=0.0283) with respect to the control. The selected medium constitutes an adequate option for the production of LEVICA-27, since it allows obtaining good cell concentration, covering the nutritional requirements and includes fewer components in its preparation. This offers economic and operational advantages for obtaining the additive at production scales. 2022 artículo científico 0864-0408 https://www.redalyc.org/articulo.oa?id=653773103003 https://www.redalyc.org/journal/6537/653773103003/ https://www.redalyc.org/journal/6537/653773103003/html/ https://www.redalyc.org/journal/6537/653773103003/653773103003.epub https://www.redalyc.org/journal/6537/653773103003/movil en http://www.redalyc.org/revista.oa?id=6537 Cuban Journal of Agricultural Science application/pdf Instituto de Ciencia Animal Cuban Journal of Agricultural Science (Cuba) Num.2 Vol.56 |
| format | Artículo científico |
| id | redalyc_653773103003 |
| institution | Redalyc |
| language | en |
| publishDate | 2022 |
| publisher | Instituto de Ciencia Animal |
| spellingShingle | Selection of a culture medium for the growth of Pichia guilliermondii LEVICA- 27 as activator additive of ruminal fermentation Bexy González Mora Estudios Agrarios yeast biomass Molasses fermentation Selection of a culture medium for the growth of Pichia guilliermondii LEVICA- 27 as activator additive of ruminal fermentation Bexy González Mora Dailyn Sosa Cossio Yoandra Marrero Rodríguez Yaneisy García Hernández Nereyda Albelo Dorta Estudios Agrarios yeast biomass Molasses fermentation In order to select a non-conventional culture medium for the growth of Pichia guilliermondii LEVICA-27 as an activator additive for ruminal fermentation, two trials were carried out in which YPG medium was used (yeast extract 10 g/L, peptone 10 g/L and glucose 20 g/L) as control medium. In the first, seven culture media were evaluated for yeast growth at 24 hours of fermentation. While in the second, growth kinetics was performed in the selected medium of the first test. In addition, in the latter, the maximum specific growth rate, the biomass doubling time, and the stoichiometric balance of the selected medium were determined. At 24 hours of fermentation, there were not differences between the media studied and the YPG medium (p=0.073). Therefore, the latter can be replaced without affecting the strain growth in 24 h. In the kinetic study, there was a higher microbial concentration at 12 h (P<0.0001) in the medium containing molasses and urea. The maximum specific growth rate was lower (P=0.0260) and the biomass doubling time was higher (P=0.0283) with respect to the control. The selected medium constitutes an adequate option for the production of LEVICA-27, since it allows obtaining good cell concentration, covering the nutritional requirements and includes fewer components in its preparation. This offers economic and operational advantages for obtaining the additive at production scales. 2022 artículo científico 0864-0408 https://www.redalyc.org/articulo.oa?id=653773103003 https://www.redalyc.org/journal/6537/653773103003/ https://www.redalyc.org/journal/6537/653773103003/html/ https://www.redalyc.org/journal/6537/653773103003/653773103003.epub https://www.redalyc.org/journal/6537/653773103003/movil en http://www.redalyc.org/revista.oa?id=6537 Cuban Journal of Agricultural Science application/pdf Instituto de Ciencia Animal Cuban Journal of Agricultural Science (Cuba) Num.2 Vol.56 |
| title | Selection of a culture medium for the growth of Pichia guilliermondii LEVICA- 27 as activator additive of ruminal fermentation |
| topic | Estudios Agrarios yeast biomass Molasses fermentation |
| url | https://www.redalyc.org/articulo.oa?id=653773103003 https://www.redalyc.org/journal/6537/653773103003/ https://www.redalyc.org/journal/6537/653773103003/html/ https://www.redalyc.org/journal/6537/653773103003/653773103003.epub https://www.redalyc.org/journal/6537/653773103003/movil |