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| Format: | Artículo científico |
| Sprache: | en |
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Universidad de Sonora
2018
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| Online-Zugang: | https://www.redalyc.org/articulo.oa?id=672971088005 |
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Inhaltsangabe:
- COMPOUNDS WITH in vitro ANTIBACTERIAL ACTIVITY FROM HYDROSOL OF Lippia palmerI AND MORPHOMETRIC CHANGES ON Listeria monocytogenes Joel Said García-Romo María Susana Yépiz-Gómez Maribel Plascencia-Jatomea Hisila Del Carmen Santacruz-Ortega Armando Burgos-Hernández Jesús Ramon García De León Francisco Javier Cinco-Moroyoqui Jesús Borboa-Flores Multidisciplinaria (Ciencias Naturales y Exactas) Lamiaceae bioactive compounds pathogenic bacteria antibacterial effect Foodborne diseases have increased, and with it, the interest to discover new natural antimicrobials; the use of ar-omatic plants, due to the extraction and use of the essential oil (EO), are widely studied and with great biological activity. However, studies with hydrosols (HS) from aromatic plants are limited. The aim in this study was to evaluate the anti-bacterial activity of HS from Lippia palmeri plant against the bacterial pathogen L. monocytogenes. HS was obtained by hydrodistillation and fractionation thereof by open column chromatography. The antibacterial activity was performed by disk diffusion. The inhibitory concentration (IC50) was estimat-ed using the PROBIT survival analysis. Ampicillin was used as control. To evaluate the diameter and cellular damage, optical microscopy and epifluorescence were used, respectively. The characterization was performed by spectroscopy. HS showed IC50 of 224 μL/mL and from HS two fractions were obtained. The fraction with the highest activity showed an IC50 of 125 mg/mL and rosmarinic acid and a phthalate derivative were identified from this fraction. HS, showed bioactivity against L. monocytogenes. Therefore, this would be a candidate for use as an active ingredient in the disinfection of food and areas in contact with them. 2018 artículo científico 1665-1456 https://www.redalyc.org/articulo.oa?id=672971088005 en http://www.redalyc.org/revista.oa?id=6729 Biotecnia application/pdf Universidad de Sonora Biotecnia (México) Num.3 Vol.20