Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad de Sonora
2024
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| _version_ | 1876443902957846528 |
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| author | Carlos Eduardo Aragón-López |
| author_facet | Carlos Eduardo Aragón-López |
| contents | Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants Carlos Eduardo Aragón-López Erika Gabriela Palomares-Reséndiz Sergio Cuevas-Tellechea Javier Rolando Reyna-Granados Beatriz Arellano-Reynoso Miguel Angel Sánchez-Castro José Clemente Leyva-Corona Marcela Ivone Morales-Pablos Multidisciplinaria (Ciencias Naturales y Exactas) LAMP ompA gene Chlamydia abortus Ovine Enzootic Abortion Ovine Enzootic Abortion (OEA) is caused by Chlamydia abortus and has recently been recognized as endemic in Mexico. Due to the impact of OEA on intensive lamb production, it is necessary to establish a sanitary control plan based on an efficient diagnosis. The loop-mediated isothermal amplification technique (LAMP) is a molecular test that can specifically identify C. abortus. The aim of this study was to standardize the LAMP technique for the specific detection of C. abortus in ovine vaginal exudates. Primers were identified and modified from an electronic database. The effective conditions of the technique were determined using synthetic DNA and positive biological samples by PCR as references. The assay detection limit was 1 × 10-5 of genetic material, equivalent to 8.5 copies. The standardized LAMP test is proposed as a novel molecular tool for the rapid and specific diagnosis of C. abortus infection from ovine vaginal exudate samples in Mexico. 2024 artículo científico 1665-1456 https://www.redalyc.org/articulo.oa?id=672978747008 https://www.redalyc.org/journal/6729/672978747008/ https://www.redalyc.org/journal/6729/672978747008/html/ https://www.redalyc.org/journal/6729/672978747008/672978747008.epub https://www.redalyc.org/journal/6729/672978747008/movil 10.18633/biotecnia.v26i1.2109 en http://www.redalyc.org/revista.oa?id=6729 Biotecnia application/pdf Universidad de Sonora Biotecnia (México) Vol.26 |
| format | Artículo científico |
| id | redalyc_672978747008 |
| institution | Redalyc |
| language | en |
| publishDate | 2024 |
| publisher | Universidad de Sonora |
| spellingShingle | Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants Carlos Eduardo Aragón-López Multidisciplinaria (Ciencias Naturales y Exactas) LAMP ompA gene Chlamydia abortus Ovine Enzootic Abortion Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants Carlos Eduardo Aragón-López Erika Gabriela Palomares-Reséndiz Sergio Cuevas-Tellechea Javier Rolando Reyna-Granados Beatriz Arellano-Reynoso Miguel Angel Sánchez-Castro José Clemente Leyva-Corona Marcela Ivone Morales-Pablos Multidisciplinaria (Ciencias Naturales y Exactas) LAMP ompA gene Chlamydia abortus Ovine Enzootic Abortion Ovine Enzootic Abortion (OEA) is caused by Chlamydia abortus and has recently been recognized as endemic in Mexico. Due to the impact of OEA on intensive lamb production, it is necessary to establish a sanitary control plan based on an efficient diagnosis. The loop-mediated isothermal amplification technique (LAMP) is a molecular test that can specifically identify C. abortus. The aim of this study was to standardize the LAMP technique for the specific detection of C. abortus in ovine vaginal exudates. Primers were identified and modified from an electronic database. The effective conditions of the technique were determined using synthetic DNA and positive biological samples by PCR as references. The assay detection limit was 1 × 10-5 of genetic material, equivalent to 8.5 copies. The standardized LAMP test is proposed as a novel molecular tool for the rapid and specific diagnosis of C. abortus infection from ovine vaginal exudate samples in Mexico. 2024 artículo científico 1665-1456 https://www.redalyc.org/articulo.oa?id=672978747008 https://www.redalyc.org/journal/6729/672978747008/ https://www.redalyc.org/journal/6729/672978747008/html/ https://www.redalyc.org/journal/6729/672978747008/672978747008.epub https://www.redalyc.org/journal/6729/672978747008/movil 10.18633/biotecnia.v26i1.2109 en http://www.redalyc.org/revista.oa?id=6729 Biotecnia application/pdf Universidad de Sonora Biotecnia (México) Vol.26 |
| title | Standardization of an isothermal test as a novel diagnostic tool in México for the molecular detection of Chlamydia abortus in small ruminants |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) LAMP ompA gene Chlamydia abortus Ovine Enzootic Abortion |
| url | https://www.redalyc.org/articulo.oa?id=672978747008 https://www.redalyc.org/journal/6729/672978747008/ https://www.redalyc.org/journal/6729/672978747008/html/ https://www.redalyc.org/journal/6729/672978747008/672978747008.epub https://www.redalyc.org/journal/6729/672978747008/movil |