Screening the microbiota of hepatopancreas associated with pirABvp toxin genes in Penaeus vannamei
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| Format: | Artículo científico |
| Language: | en |
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Universidad de Sonora
2024
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| author | Luz Angélica Avila-Villa |
| author_facet | Luz Angélica Avila-Villa |
| contents | Screening the microbiota of hepatopancreas associated with pirABvp toxin genes in Penaeus vannamei Luz Angélica Avila-Villa Gabriel Amílcar Barco-Mendoza Roberto Rodríguez-Ramírez Jesus Daniel Villanueva-Zayas Marcel Martínez-Porchas Alma Guadalupe Villa-Lerma Belinda Vallejo-Córdoba Josué Delgado-Domínguez Multidisciplinaria (Ciencias Naturales y Exactas) next AHPND Vibrio shrimp PirABvp Shrimp aquaculture has rapidly expanded in the last decades, representing an important economic activity worldwide; however, infections (bacterial and viral) are one of the biggest challenges in aquaculture activity. Acute Hepatopancreatic Necrosis Disease (AHPND) is a bacterial disease that affects shrimp farm ponds and occurs during the first 30 days after stocking in shrimp ponds, causing mortalities of up to 70 - 100 %. Microbiota has an important role in shrimp aquaculture and disease control. In the present study, pirA and pirB genes were amplified by PCR to confirm AHPND and non-AHPND in shrimp hepatopancreas; samples were sequenced using the MiSeq platform targeting the V3-V4 16S ribosomal (rRNA) hypervariable regions. Results demonstrated a diverse microbiota in the non-AHPND group, mainly composed of phyla Proteobacteria, Firmicutes, and Actinobacteria. Furthermore, Proteobacteria was the dominant phyla in the hepatopancreas infected with AHPND, while Firmicutes tended to decrease. Vibrio was the most abundant at the genus level, prevailing in some genera like Corynebacterium, Weissella, Lactobacillus, Photobacterium, and Geobacillus. Results suggest that AHPND causes dysbiosis in the hepatopancreatic microbiota, and the Firmicutes phylum could require to be restored under such scenario. 2024 artículo científico 1665-1456 https://www.redalyc.org/articulo.oa?id=672978747013 https://www.redalyc.org/journal/6729/672978747013/ https://www.redalyc.org/journal/6729/672978747013/html/ https://www.redalyc.org/journal/6729/672978747013/672978747013.epub https://www.redalyc.org/journal/6729/672978747013/movil 10.18633/biotecnia.v26i1.2181 en http://www.redalyc.org/revista.oa?id=6729 Biotecnia application/pdf Universidad de Sonora Biotecnia (México) Vol.26 |
| format | Artículo científico |
| id | redalyc_672978747013 |
| institution | Redalyc |
| language | en |
| publishDate | 2024 |
| publisher | Universidad de Sonora |
| spellingShingle | Screening the microbiota of hepatopancreas associated with pirABvp toxin genes in Penaeus vannamei Luz Angélica Avila-Villa Multidisciplinaria (Ciencias Naturales y Exactas) next AHPND Vibrio shrimp PirABvp Screening the microbiota of hepatopancreas associated with pirABvp toxin genes in Penaeus vannamei Luz Angélica Avila-Villa Gabriel Amílcar Barco-Mendoza Roberto Rodríguez-Ramírez Jesus Daniel Villanueva-Zayas Marcel Martínez-Porchas Alma Guadalupe Villa-Lerma Belinda Vallejo-Córdoba Josué Delgado-Domínguez Multidisciplinaria (Ciencias Naturales y Exactas) next AHPND Vibrio shrimp PirABvp Shrimp aquaculture has rapidly expanded in the last decades, representing an important economic activity worldwide; however, infections (bacterial and viral) are one of the biggest challenges in aquaculture activity. Acute Hepatopancreatic Necrosis Disease (AHPND) is a bacterial disease that affects shrimp farm ponds and occurs during the first 30 days after stocking in shrimp ponds, causing mortalities of up to 70 - 100 %. Microbiota has an important role in shrimp aquaculture and disease control. In the present study, pirA and pirB genes were amplified by PCR to confirm AHPND and non-AHPND in shrimp hepatopancreas; samples were sequenced using the MiSeq platform targeting the V3-V4 16S ribosomal (rRNA) hypervariable regions. Results demonstrated a diverse microbiota in the non-AHPND group, mainly composed of phyla Proteobacteria, Firmicutes, and Actinobacteria. Furthermore, Proteobacteria was the dominant phyla in the hepatopancreas infected with AHPND, while Firmicutes tended to decrease. Vibrio was the most abundant at the genus level, prevailing in some genera like Corynebacterium, Weissella, Lactobacillus, Photobacterium, and Geobacillus. Results suggest that AHPND causes dysbiosis in the hepatopancreatic microbiota, and the Firmicutes phylum could require to be restored under such scenario. 2024 artículo científico 1665-1456 https://www.redalyc.org/articulo.oa?id=672978747013 https://www.redalyc.org/journal/6729/672978747013/ https://www.redalyc.org/journal/6729/672978747013/html/ https://www.redalyc.org/journal/6729/672978747013/672978747013.epub https://www.redalyc.org/journal/6729/672978747013/movil 10.18633/biotecnia.v26i1.2181 en http://www.redalyc.org/revista.oa?id=6729 Biotecnia application/pdf Universidad de Sonora Biotecnia (México) Vol.26 |
| title | Screening the microbiota of hepatopancreas associated with pirABvp toxin genes in Penaeus vannamei |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) next AHPND Vibrio shrimp PirABvp |
| url | https://www.redalyc.org/articulo.oa?id=672978747013 https://www.redalyc.org/journal/6729/672978747013/ https://www.redalyc.org/journal/6729/672978747013/html/ https://www.redalyc.org/journal/6729/672978747013/672978747013.epub https://www.redalyc.org/journal/6729/672978747013/movil |