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| Format: | Artículo científico |
| Language: | en |
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Colegio de Postgraduados
2006
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| Online Access: | https://www.redalyc.org/articulo.oa?id=68518204 |
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Table of Contents:
- Effect of culture media and fermentation parameters on phytase production by the thermophilic fungus Myceliophthora thermophila in solid state fermentation H. Hassouni M. Ismaili Alaoui I. Gaime Perraud C. Augur S. Roussos Biología CaCl2 MgSO4 Phytase carbon source nitrogen source Phytase production by thermophilic filamentous fungi was evaluated in solid statefermentation. A total of twenty strains belonging to Rhizomucor spp. (9), Myceliophthorathermophila (6), Thermoascus aurantiacus (4), and Paecilomyces variotii (1) were screened.Sugar cane bagasse was used as support for assessing the effect of different carbon andnitrogen sources, as well as mineral salts. Solid state fermentation parameters (initial moisture,initial pH, aeration rate) were also studied. Myceliophthora thermophila showed higher phytaseactivity and was selected for further studies. Synthetic culture media containing differentcarbon and nitrogen sources were tested for phytase production by this species in solid statefermentation. M. thermophila showed the highest phytase activity after 36 h of fermentation,when grown on a synthetic culture medium (glucose 10 g/L; phytic acid 2 g/L). (NH4)2HPO4,CaCl2, and MgSO4 favored high phytase activity in M. thermophila. Optimal initial cultureconditions for phytase production were determined (pH, 6.0; moisture, 75%; aeration rate, 25ml/min/column). In comparison with Aspergillus ficuum, the phytase activity of M. thermophilawas 2.5 times higher. Phytase was most active at pH 5.5 and 45-50 C, but it was also active at70 C. Optimization studies resulted in a 4-fold increase of phytase activity. 2006 artículo científico 1534-2581 https://www.redalyc.org/articulo.oa?id=68518204 en http://www.redalyc.org/revista.oa?id=685 Micología Aplicada International application/pdf Colegio de Postgraduados Micología Aplicada International (México) Num.2 Vol.18