KINETIC STUDIES OF INVERTASE PRODUCTION BY XEROPHILIC ASPERGILLUS AND PENICILLIUM STRAINS UNDER SUBMERGED CULTURE
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| Natura: | Artículo científico |
| Lingua: | en |
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Colegio de Postgraduados
2011
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| author | F. Veana |
| author_facet | F. Veana |
| contents | KINETIC STUDIES OF INVERTASE PRODUCTION BY XEROPHILIC ASPERGILLUS AND PENICILLIUM STRAINS UNDER SUBMERGED CULTURE F. Veana C. N. Aguilar R. Rodríguez Herrera Biología radial growth submerged culture fructofuranosidase invertase activity kinetic parameters Invertase catalyzes the hydrolysis of sucrose into glucose and fructose. This enzyme is important in the food industry. Several fungal species have been used for invertase production, most of them isolated from tropical areas. However, there are xerophilic strains capable of increasing the amount of enzyme produced. In this study, four xerophilic fungal strains (Penicillium pinophilum EH2, P. purpurogenum GH2, P. citrinum ESS, Aspergillus niger GH1) isolated from a Mexican semi-desert were used for invertase production in submerged culture. The strain GH1 of A. niger showed higher specific growth rate than Penicillium strains. This strain produced extracellular invertase with the highest enzymatic activity (8,625 U/L) after 48 h. The strain ESS of P. citrinum showed the maximum invertase activity (2,308 U/L) after 72 h. Xerophilic strains tolerated pH changes during submerged culture. The specific rate of substrate absorption, specific rate of product formation, productivity and enzyme yield of the strain GH1 of A. niger were higher than those observed for Penicillium strains. Results suggested that this xerophilic strain is an alternative for invertase production. 2011 artículo científico 1534-2581 https://www.redalyc.org/articulo.oa?id=68519085001 en http://www.redalyc.org/revista.oa?id=685 Micología Aplicada International application/pdf Colegio de Postgraduados Micología Aplicada International (México) Num.2 Vol.23 |
| format | Artículo científico |
| id | redalyc_68519085001 |
| language | en |
| publishDate | 2011 |
| publisher | Colegio de Postgraduados |
| spellingShingle | KINETIC STUDIES OF INVERTASE PRODUCTION BY XEROPHILIC ASPERGILLUS AND PENICILLIUM STRAINS UNDER SUBMERGED CULTURE F. Veana Biología radial growth submerged culture fructofuranosidase invertase activity kinetic parameters KINETIC STUDIES OF INVERTASE PRODUCTION BY XEROPHILIC ASPERGILLUS AND PENICILLIUM STRAINS UNDER SUBMERGED CULTURE F. Veana C. N. Aguilar R. Rodríguez Herrera Biología radial growth submerged culture fructofuranosidase invertase activity kinetic parameters Invertase catalyzes the hydrolysis of sucrose into glucose and fructose. This enzyme is important in the food industry. Several fungal species have been used for invertase production, most of them isolated from tropical areas. However, there are xerophilic strains capable of increasing the amount of enzyme produced. In this study, four xerophilic fungal strains (Penicillium pinophilum EH2, P. purpurogenum GH2, P. citrinum ESS, Aspergillus niger GH1) isolated from a Mexican semi-desert were used for invertase production in submerged culture. The strain GH1 of A. niger showed higher specific growth rate than Penicillium strains. This strain produced extracellular invertase with the highest enzymatic activity (8,625 U/L) after 48 h. The strain ESS of P. citrinum showed the maximum invertase activity (2,308 U/L) after 72 h. Xerophilic strains tolerated pH changes during submerged culture. The specific rate of substrate absorption, specific rate of product formation, productivity and enzyme yield of the strain GH1 of A. niger were higher than those observed for Penicillium strains. Results suggested that this xerophilic strain is an alternative for invertase production. 2011 artículo científico 1534-2581 https://www.redalyc.org/articulo.oa?id=68519085001 en http://www.redalyc.org/revista.oa?id=685 Micología Aplicada International application/pdf Colegio de Postgraduados Micología Aplicada International (México) Num.2 Vol.23 |
| title | KINETIC STUDIES OF INVERTASE PRODUCTION BY XEROPHILIC ASPERGILLUS AND PENICILLIUM STRAINS UNDER SUBMERGED CULTURE |
| topic | Biología radial growth submerged culture fructofuranosidase invertase activity kinetic parameters |
| url | https://www.redalyc.org/articulo.oa?id=68519085001 |