In vitro growth of Ceratocystis paradoxa in oil palm (Elaeis guineensis) fruit extract media
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| Format: | Artículo científico |
| Langue: | en |
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Colegio de Postgraduados
2007
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| _version_ | 1876488356032937984 |
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| author | F. I. Okungbowa |
| author_facet | F. I. Okungbowa |
| contents | In vitro growth of Ceratocystis paradoxa in oil palm (Elaeis guineensis) fruit extract media F. I. Okungbowa A. P. O. Dede Biología Nigeria oil palm fruit extract in vitro growth Ceratocystis paradoxa The fungus Ceratocystis paradoxa, a pathogen of the oil palm and other economiccrops, grows well in vitro on the oil palm fruit extract media (solid and liquidforms). A stock culture of the fungus (IMI specimen no. 79166) was used in thisstudy, and its growth in oil palm fruit basal medium, potato dextrose and corn mealmedia were compared at three different temperatures (15 C, 28±2 C, 35 C). After72 h of incubation at room temperature (28±2 C), the fungus had equal mean radialgrowth on potato dextrose agar (PDA) and oil palm fruit extract agar (PFEA) witha growth increase of 38 mm, while an increase of 11 mm was recorded for corn mealagar (CMA). At 15 C, growth was generally slow but the highest mean radial growth(increase of 10.8 mm) was recorded for PFEA, while 3.2 mm and 6.0 mm for CMAand PDA, respectively. No growth was observed on the three solid media at 35 C.However, the fungus grew at 35 C with mean increases in dry weight of 197 mg, 56 mg,and 271 mg, for potato dextrose broth (PDB), corn meal broth (CMB) and palm fruitextract broth (PFEB), respectively. PFEB yielded the highest mycelial dry weight of 80mg at 15 C. There were signifi cant differences between mycelial dry weight in PFEBand the control at all temperatures, as well as between PFEB and the other media atthe same temperature. Palm fruit extract can be used as a substitute for commercialmedia for in vitro growth of this fungus. 2007 artículo científico 1534-2581 https://www.redalyc.org/articulo.oa?id=68519203 en http://www.redalyc.org/revista.oa?id=685 Micología Aplicada International application/pdf Colegio de Postgraduados Micología Aplicada International (México) Num.2 Vol.19 |
| format | Artículo científico |
| id | redalyc_68519203 |
| institution | Redalyc |
| language | en |
| publishDate | 2007 |
| publisher | Colegio de Postgraduados |
| spellingShingle | In vitro growth of Ceratocystis paradoxa in oil palm (Elaeis guineensis) fruit extract media F. I. Okungbowa Biología Nigeria oil palm fruit extract in vitro growth Ceratocystis paradoxa In vitro growth of Ceratocystis paradoxa in oil palm (Elaeis guineensis) fruit extract media F. I. Okungbowa A. P. O. Dede Biología Nigeria oil palm fruit extract in vitro growth Ceratocystis paradoxa The fungus Ceratocystis paradoxa, a pathogen of the oil palm and other economiccrops, grows well in vitro on the oil palm fruit extract media (solid and liquidforms). A stock culture of the fungus (IMI specimen no. 79166) was used in thisstudy, and its growth in oil palm fruit basal medium, potato dextrose and corn mealmedia were compared at three different temperatures (15 C, 28±2 C, 35 C). After72 h of incubation at room temperature (28±2 C), the fungus had equal mean radialgrowth on potato dextrose agar (PDA) and oil palm fruit extract agar (PFEA) witha growth increase of 38 mm, while an increase of 11 mm was recorded for corn mealagar (CMA). At 15 C, growth was generally slow but the highest mean radial growth(increase of 10.8 mm) was recorded for PFEA, while 3.2 mm and 6.0 mm for CMAand PDA, respectively. No growth was observed on the three solid media at 35 C.However, the fungus grew at 35 C with mean increases in dry weight of 197 mg, 56 mg,and 271 mg, for potato dextrose broth (PDB), corn meal broth (CMB) and palm fruitextract broth (PFEB), respectively. PFEB yielded the highest mycelial dry weight of 80mg at 15 C. There were signifi cant differences between mycelial dry weight in PFEBand the control at all temperatures, as well as between PFEB and the other media atthe same temperature. Palm fruit extract can be used as a substitute for commercialmedia for in vitro growth of this fungus. 2007 artículo científico 1534-2581 https://www.redalyc.org/articulo.oa?id=68519203 en http://www.redalyc.org/revista.oa?id=685 Micología Aplicada International application/pdf Colegio de Postgraduados Micología Aplicada International (México) Num.2 Vol.19 |
| title | In vitro growth of Ceratocystis paradoxa in oil palm (Elaeis guineensis) fruit extract media |
| topic | Biología Nigeria oil palm fruit extract in vitro growth Ceratocystis paradoxa |
| url | https://www.redalyc.org/articulo.oa?id=68519203 |