Submerged culture for production of ellagic acid from pomegranate husk by Aspergillus niger GH1
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| Natura: | Artículo científico |
| Lingua: | en |
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Colegio de Postgraduados
2014
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| _version_ | 1876490469875122176 |
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| author | L. Sepúlveda |
| author_facet | L. Sepúlveda |
| contents | Submerged culture for production of ellagic acid from pomegranate husk by Aspergillus niger GH1 L. Sepúlveda J. J. Buenrostro -Figueroa J A. Ascacio-Valdés A. F. Aguilera-Carbó R. Rodríguez -Herrera J. C. Contreras-Esquivel C. N. Aguilar Biología Box ellagitannase ellagitannins Behnken design Aspergillus niger Ellagic acid is a bioactive compound derived from the hydrolysis of the ellagitannins. Ellagic acid has important applications in the food, pharmaceutical and cosmetic industry due its beneficial biological properties. Ellagic acid has been obtained from several sources, such as some berries, semi-desert plants, and oak trees. Pomegranate husk has a high content of ellagitannins, which can be bio-converted into ellagic acid by fungal bioprocesses, although there is no industrial biotechnological process available. The aim of this work was to find appropriate conditions for ellagic acid production by Aspergillus niger GH1 in submerged culture using pomegranate husk powder. The influence of culture factors was studied by a Box-Bhenken design. The maximum ellagic acid production was 21.19 mg g -1 of pomegranate husk powder. Substrate and pH influenced the ellagic acid production. A constant consumption was present in the substrate during the kinetics of hydrolysable tannins (ellagitannins). The maximum protein accumulation was reached at 24 h, which can be associated with the activity of some enzymes present in the culture. The best conditions for ellagic acid production in submerged culture were: substrate level of 7.5 g L -1 , pH of 5.5, and agitation of 150 rpm. The developed system represents an alternative for large-scale ellagic acid production. 2014 artículo científico 1534-2581 https://www.redalyc.org/articulo.oa?id=68531695002 en http://www.redalyc.org/revista.oa?id=685 Micología Aplicada International application/pdf Colegio de Postgraduados Micología Aplicada International (México) Num.2 Vol.26 |
| format | Artículo científico |
| id | redalyc_68531695002 |
| institution | Redalyc |
| language | en |
| publishDate | 2014 |
| publisher | Colegio de Postgraduados |
| spellingShingle | Submerged culture for production of ellagic acid from pomegranate husk by Aspergillus niger GH1 L. Sepúlveda Biología Box ellagitannase ellagitannins Behnken design Aspergillus niger Submerged culture for production of ellagic acid from pomegranate husk by Aspergillus niger GH1 L. Sepúlveda J. J. Buenrostro -Figueroa J A. Ascacio-Valdés A. F. Aguilera-Carbó R. Rodríguez -Herrera J. C. Contreras-Esquivel C. N. Aguilar Biología Box ellagitannase ellagitannins Behnken design Aspergillus niger Ellagic acid is a bioactive compound derived from the hydrolysis of the ellagitannins. Ellagic acid has important applications in the food, pharmaceutical and cosmetic industry due its beneficial biological properties. Ellagic acid has been obtained from several sources, such as some berries, semi-desert plants, and oak trees. Pomegranate husk has a high content of ellagitannins, which can be bio-converted into ellagic acid by fungal bioprocesses, although there is no industrial biotechnological process available. The aim of this work was to find appropriate conditions for ellagic acid production by Aspergillus niger GH1 in submerged culture using pomegranate husk powder. The influence of culture factors was studied by a Box-Bhenken design. The maximum ellagic acid production was 21.19 mg g -1 of pomegranate husk powder. Substrate and pH influenced the ellagic acid production. A constant consumption was present in the substrate during the kinetics of hydrolysable tannins (ellagitannins). The maximum protein accumulation was reached at 24 h, which can be associated with the activity of some enzymes present in the culture. The best conditions for ellagic acid production in submerged culture were: substrate level of 7.5 g L -1 , pH of 5.5, and agitation of 150 rpm. The developed system represents an alternative for large-scale ellagic acid production. 2014 artículo científico 1534-2581 https://www.redalyc.org/articulo.oa?id=68531695002 en http://www.redalyc.org/revista.oa?id=685 Micología Aplicada International application/pdf Colegio de Postgraduados Micología Aplicada International (México) Num.2 Vol.26 |
| title | Submerged culture for production of ellagic acid from pomegranate husk by Aspergillus niger GH1 |
| topic | Biología Box ellagitannase ellagitannins Behnken design Aspergillus niger |
| url | https://www.redalyc.org/articulo.oa?id=68531695002 |