Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration
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| Format: | Artículo científico |
| Language: | en |
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Universidad de Córdoba
2014
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| author | Paula Díaz T |
| author_facet | Paula Díaz T |
| contents | Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration Paula Díaz T Adriana Bernal G Camilo López C Veterinaria beta Cassava transient gene expression Agrobacterium tumefaciens glucuronidase ( Source: NAL USDA ) Objective. Assess transient gene expression of GUS in cassava ( Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A. tumefaciens strains GV3101 and AGL1 containing pCAMBIA1305.2 were used to evaluate transient gene expression of β- glucuronidase ( GUS ). A. tumefaciens infiltration (agroinfiltration) was made using both leaves from in vitro and 1 month old greenhouse plants. Leaves were incubated in X-GLUC buffer, stained and photographed to detect GUS activity. Results. Agroinfiltration assays showed GUS transient expression in leaves of cassava varieties widely cultivated in the north coast and eastern savannah, MCOL2215 (Venezuelan) and CM6438-14 (Vergara), respectively. A. tumefaciens agressive strain AGL1 showed high efficiency inducing GUS expression in cassava leaves. Conclusions. We recommend using A. tumefaciens agressive strain AGL1 for agroinfiltration to assess transient expression in cassava leaves. 2014 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69332509014 en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.3 Vol.19 |
| format | Artículo científico |
| id | redalyc_69332509014 |
| institution | Redalyc |
| language | en |
| publishDate | 2014 |
| publisher | Universidad de Córdoba |
| spellingShingle | Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration Paula Díaz T Veterinaria beta Cassava transient gene expression Agrobacterium tumefaciens glucuronidase ( Source: NAL USDA ) Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration Paula Díaz T Adriana Bernal G Camilo López C Veterinaria beta Cassava transient gene expression Agrobacterium tumefaciens glucuronidase ( Source: NAL USDA ) Objective. Assess transient gene expression of GUS in cassava ( Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A. tumefaciens strains GV3101 and AGL1 containing pCAMBIA1305.2 were used to evaluate transient gene expression of β- glucuronidase ( GUS ). A. tumefaciens infiltration (agroinfiltration) was made using both leaves from in vitro and 1 month old greenhouse plants. Leaves were incubated in X-GLUC buffer, stained and photographed to detect GUS activity. Results. Agroinfiltration assays showed GUS transient expression in leaves of cassava varieties widely cultivated in the north coast and eastern savannah, MCOL2215 (Venezuelan) and CM6438-14 (Vergara), respectively. A. tumefaciens agressive strain AGL1 showed high efficiency inducing GUS expression in cassava leaves. Conclusions. We recommend using A. tumefaciens agressive strain AGL1 for agroinfiltration to assess transient expression in cassava leaves. 2014 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69332509014 en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.3 Vol.19 |
| title | Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration |
| topic | Veterinaria beta Cassava transient gene expression Agrobacterium tumefaciens glucuronidase ( Source: NAL USDA ) |
| url | https://www.redalyc.org/articulo.oa?id=69332509014 |