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| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidad de Córdoba
2015
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| Online Access: | https://www.redalyc.org/articulo.oa?id=69341382002 |
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Table of Contents:
- Genetic variability of broodstocks of restocking programs in Brazil Nelson Lopera-Barrero Ed Lima Luiz Filho Elenice Goes Pedro Castro Aline Zardin Angela Poveda-Parra Ricardo Ribeiro Veterinaria Rhamdia quelen CAB Thesaurus) Leporinus elongatus Genetic conservation Pseudoplatystoma fasciatum Objective. The aim of this study was evaluate the genetic diversity of the following broodstocks: piapara (Leporinus elongatus), dourado (Salminus brasiliensis), jundiá (Rhamdia quelen) and cachara (Pseudoplatystoma fasciatum) already useful for restocking programs in the Paranapanema, Iguaçu and Paraná Brazilian Rivers. Materials and methods. Samples from the caudal fin of 122 fish were analyzed. DNA was extracted by NaCl protocol. PCR products were separated by a horizontal agarose gel electrophoresis. The fragments were visualized by staining with ethidium bromide. Results. The amplification of 25 primers generated different fragments in studied species that allowed characterizing 440 fragments of 100-2900 bp. High percentage of polymorphic fragments (66.67 to 86.29), Shannon index (0.365 to 0.486) and genetic diversity of Nei (0.248 to 0.331) were detected. Conclusions. The level of genetic variability in the broodstocks was adequate for allowing their use in restocking programs in the studied Rivers. However, periodical monitoring studies of genetic variability in these stocks, the mating system, reproductive system and general management must be made to guarantee the preservation of wild populations. 2015 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69341382002 en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.3 Vol.20