First approach to molecular epidemiology of bovine tuberculosis in Colombia

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Main Author: Jimena Jojoa-Jojoa
Format: Artículo científico
Language:en
Published: Universidad de Córdoba 2016
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author Jimena Jojoa-Jojoa
author_facet Jimena Jojoa-Jojoa
contents First approach to molecular epidemiology of bovine tuberculosis in Colombia Jimena Jojoa-Jojoa Maira Wintaco M Francisco Osorio R Gloria Puerto-Castro Martha Guerrero-Guerrero Veterinaria MeSH) diagnostic Bovine tuberculosis Mycobacterium bovis molecular epidemiology Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health. 2016 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69343172013 https://www.redalyc.org/journal/693/69343172013/ https://www.redalyc.org/journal/693/69343172013/html/ https://www.redalyc.org/journal/693/69343172013/69343172013.epub https://www.redalyc.org/journal/693/69343172013/movil en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.1 Vol.21
format Artículo científico
id redalyc_69343172013
institution Redalyc
language en
publishDate 2016
publisher Universidad de Córdoba
spellingShingle First approach to molecular epidemiology of bovine tuberculosis in Colombia
Jimena Jojoa-Jojoa
Veterinaria
MeSH)
diagnostic
Bovine tuberculosis
Mycobacterium bovis
molecular epidemiology
First approach to molecular epidemiology of bovine tuberculosis in Colombia Jimena Jojoa-Jojoa Maira Wintaco M Francisco Osorio R Gloria Puerto-Castro Martha Guerrero-Guerrero Veterinaria MeSH) diagnostic Bovine tuberculosis Mycobacterium bovis molecular epidemiology Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health. 2016 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69343172013 https://www.redalyc.org/journal/693/69343172013/ https://www.redalyc.org/journal/693/69343172013/html/ https://www.redalyc.org/journal/693/69343172013/69343172013.epub https://www.redalyc.org/journal/693/69343172013/movil en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.1 Vol.21
title First approach to molecular epidemiology of bovine tuberculosis in Colombia
topic Veterinaria
MeSH)
diagnostic
Bovine tuberculosis
Mycobacterium bovis
molecular epidemiology
url https://www.redalyc.org/articulo.oa?id=69343172013
https://www.redalyc.org/journal/693/69343172013/
https://www.redalyc.org/journal/693/69343172013/html/
https://www.redalyc.org/journal/693/69343172013/69343172013.epub
https://www.redalyc.org/journal/693/69343172013/movil