Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans
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Universidad de Córdoba
2016
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| author | Joan Salas-Leiva |
| author_facet | Joan Salas-Leiva |
| contents | Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans Joan Salas-Leiva Enrique Dupré M Dayana Salas-Leiva Veterinaria DeCS) Diatom freezing nutrients storage viability (Source: AGROVOG Objective. The effect of cryopreservation on the proximate composition of microalgae Chaetoceros calcitrans was evaluated. Materials and methods. C. calcitrans was cultured and cryopreserved using 5% (v/v) dimethylsulfoxide as cryoprotectant. The freezing was controlled at a rate of 3°C/min, up to -60°C and then the microalgae were immersed in liquid nitrogen (-196°C). After storage in liquid nitrogen, microalgae were rapidly thawed out and subcultured. The percentage of proteins, lipids and carbohydrates was analyzed using absorption spectrophotometry and the organic matter was studied by gravimetric analysis. Results. There was no significant variation between the proximate composition of C. calcitrans cryopreserved and the controls (p>0.05). Conclusion. Our results show that, despite low cell recovery after the preservation of this organism at low temperatures, there is no apparent loss of nutritional characteristics caused by the storing process at low temperatures. 2016 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69343172016 https://www.redalyc.org/journal/693/69343172016/ https://www.redalyc.org/journal/693/69343172016/html/ https://www.redalyc.org/journal/693/69343172016/69343172016.epub https://www.redalyc.org/journal/693/69343172016/movil en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.1 Vol.21 |
| format | Artículo científico |
| id | redalyc_69343172016 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Universidad de Córdoba |
| spellingShingle | Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans Joan Salas-Leiva Veterinaria DeCS) Diatom freezing nutrients storage viability (Source: AGROVOG Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans Joan Salas-Leiva Enrique Dupré M Dayana Salas-Leiva Veterinaria DeCS) Diatom freezing nutrients storage viability (Source: AGROVOG Objective. The effect of cryopreservation on the proximate composition of microalgae Chaetoceros calcitrans was evaluated. Materials and methods. C. calcitrans was cultured and cryopreserved using 5% (v/v) dimethylsulfoxide as cryoprotectant. The freezing was controlled at a rate of 3°C/min, up to -60°C and then the microalgae were immersed in liquid nitrogen (-196°C). After storage in liquid nitrogen, microalgae were rapidly thawed out and subcultured. The percentage of proteins, lipids and carbohydrates was analyzed using absorption spectrophotometry and the organic matter was studied by gravimetric analysis. Results. There was no significant variation between the proximate composition of C. calcitrans cryopreserved and the controls (p>0.05). Conclusion. Our results show that, despite low cell recovery after the preservation of this organism at low temperatures, there is no apparent loss of nutritional characteristics caused by the storing process at low temperatures. 2016 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69343172016 https://www.redalyc.org/journal/693/69343172016/ https://www.redalyc.org/journal/693/69343172016/html/ https://www.redalyc.org/journal/693/69343172016/69343172016.epub https://www.redalyc.org/journal/693/69343172016/movil en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.1 Vol.21 |
| title | Proximate composition analysis posterior to the cryopreservation of Chaetoceros calcitrans |
| topic | Veterinaria DeCS) Diatom freezing nutrients storage viability (Source: AGROVOG |
| url | https://www.redalyc.org/articulo.oa?id=69343172016 https://www.redalyc.org/journal/693/69343172016/ https://www.redalyc.org/journal/693/69343172016/html/ https://www.redalyc.org/journal/693/69343172016/69343172016.epub https://www.redalyc.org/journal/693/69343172016/movil |