In vitro digestibility of specific dsRNA by enzymes of digestive tract of shrimp Litopenaeus vannamei
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| Natura: | Artículo científico |
| Lingua: | en |
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Universidad de Córdoba
2017
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| _version_ | 1876446950840074240 |
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| author | Ana R Álvarez-Sánchez |
| author_facet | Ana R Álvarez-Sánchez |
| contents | In vitro digestibility of specific dsRNA by enzymes of digestive tract of shrimp Litopenaeus vannamei Ana R Álvarez-Sánchez Héctor Nolasco-Soria Humberto Mejía-Ruíz Veterinaria WSSV WSSV Aquaculture Aquaculture balanced feed Objective. The digestibility of specific dsRNA by action of the enzymes of digestive tract of the whiteleg shrimp Litopenaeus vannamei was determined in vitro. Materials and methods. Digestive enzyme activity (amylase, lipase, protease, DNase and RNase) was measured in the stomach, digestive gland, and anterior, middle, and posterior intestine of juvenile shrimp, and the digestibility of DNA, RNA and the dsRNA-ORF89, specific to WSSV, was determined by in vitro assays, as well as electrophoretic and densitometric analyses. Results. The highest enzymatic activity was found in the digestive gland: amylase (81.41%), lipase (92.60%), protease (78.20%), DNase (90.85%), and RNase (93.14%). The highest digestive capacity against DNA, RNA, and dsRNA was found in the digestive gland (5.11 ng of DNA per minute, 8.55 ng of RNA per minute, and 1.48 ng dsRNA per minute). Conclusions. The highest digestibility of dsRNA-ORF89, specific to WSSV, was found in the digestive gland, whereas the lowest digestibility was observed in the posterior intestine. This is the first report regarding the digestibility of dsRNA-ORF89 by whiteleg shrimp digestive tract enzymes, with potential therapeutic importance in shrimp culture to prevent WSSV disease through balanced feed. 2017 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69353272006 https://www.redalyc.org/journal/693/69353272006/ https://www.redalyc.org/journal/693/69353272006/html/ https://www.redalyc.org/journal/693/69353272006/69353272006.epub https://www.redalyc.org/journal/693/69353272006/movil en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.2 Vol.22 |
| format | Artículo científico |
| id | redalyc_69353272006 |
| institution | Redalyc |
| language | en |
| publishDate | 2017 |
| publisher | Universidad de Córdoba |
| spellingShingle | In vitro digestibility of specific dsRNA by enzymes of digestive tract of shrimp Litopenaeus vannamei Ana R Álvarez-Sánchez Veterinaria WSSV WSSV Aquaculture Aquaculture balanced feed In vitro digestibility of specific dsRNA by enzymes of digestive tract of shrimp Litopenaeus vannamei Ana R Álvarez-Sánchez Héctor Nolasco-Soria Humberto Mejía-Ruíz Veterinaria WSSV WSSV Aquaculture Aquaculture balanced feed Objective. The digestibility of specific dsRNA by action of the enzymes of digestive tract of the whiteleg shrimp Litopenaeus vannamei was determined in vitro. Materials and methods. Digestive enzyme activity (amylase, lipase, protease, DNase and RNase) was measured in the stomach, digestive gland, and anterior, middle, and posterior intestine of juvenile shrimp, and the digestibility of DNA, RNA and the dsRNA-ORF89, specific to WSSV, was determined by in vitro assays, as well as electrophoretic and densitometric analyses. Results. The highest enzymatic activity was found in the digestive gland: amylase (81.41%), lipase (92.60%), protease (78.20%), DNase (90.85%), and RNase (93.14%). The highest digestive capacity against DNA, RNA, and dsRNA was found in the digestive gland (5.11 ng of DNA per minute, 8.55 ng of RNA per minute, and 1.48 ng dsRNA per minute). Conclusions. The highest digestibility of dsRNA-ORF89, specific to WSSV, was found in the digestive gland, whereas the lowest digestibility was observed in the posterior intestine. This is the first report regarding the digestibility of dsRNA-ORF89 by whiteleg shrimp digestive tract enzymes, with potential therapeutic importance in shrimp culture to prevent WSSV disease through balanced feed. 2017 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69353272006 https://www.redalyc.org/journal/693/69353272006/ https://www.redalyc.org/journal/693/69353272006/html/ https://www.redalyc.org/journal/693/69353272006/69353272006.epub https://www.redalyc.org/journal/693/69353272006/movil en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.2 Vol.22 |
| title | In vitro digestibility of specific dsRNA by enzymes of digestive tract of shrimp Litopenaeus vannamei |
| topic | Veterinaria WSSV WSSV Aquaculture Aquaculture balanced feed |
| url | https://www.redalyc.org/articulo.oa?id=69353272006 https://www.redalyc.org/journal/693/69353272006/ https://www.redalyc.org/journal/693/69353272006/html/ https://www.redalyc.org/journal/693/69353272006/69353272006.epub https://www.redalyc.org/journal/693/69353272006/movil |