Molecular identification of tick-borne hemoparasites in equines from Northwestern Colombia
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| Format: | Artículo científico |
| Language: | en |
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Universidad de Córdoba
2017
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| _version_ | 1876452387842949120 |
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| author | Yeison Agudelo-Ruíz |
| author_facet | Yeison Agudelo-Ruíz |
| contents | Molecular identification of tick-borne hemoparasites in equines from Northwestern Colombia Yeison Agudelo-Ruíz Leidy Acevedo-Gutiérrez Andrés Montoya-Sanchéz Luis Paternina Veterinaria PCR Babesia Anaplasma Ehrlichia Theileria Objective. To detect and identify Anaplasmataceae agents and piroplasms in equines from the slaughterhouse “La Rinconada” at Rionegro municipality in Antioquia. Materials and methods. A descriptive cross-sectional study was carried out on equines selected by convenience during a period of 2015. Information about species, sex, age and origin of the animals. Whole blood was collected for DNA extraction procedure, and a PCR targeting a 360bp of Anaplasmataceae 16S ribosomal gene and 450bp of 18S ribosomal gene of Piroplasm were used for detection. PCR amplicons selected were submitted to direct sequencing for identification of hemoparasites through genetic analysis Results.135 equine samples from Antioquia, Cordoba y Sucre were analyzed. 78% were horses, 16% were donkeys and 6% were mules. Anaplasmataceae agents were not detected in any sample, meanwhile 13% were positive to piroplasm PCR. Sequence analysis reveals the circulation of Theileria equi in northwestern Colombia. Conclusions. This work presents the first molecular evidence of at least three genotypes of T. equi in equines of northwestern Colombia. 2017 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69353273003 https://www.redalyc.org/journal/693/69353273003/ https://www.redalyc.org/journal/693/69353273003/html/ https://www.redalyc.org/journal/693/69353273003/69353273003.epub https://www.redalyc.org/journal/693/69353273003/movil en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Vol.22 |
| format | Artículo científico |
| id | redalyc_69353273003 |
| institution | Redalyc |
| language | en |
| publishDate | 2017 |
| publisher | Universidad de Córdoba |
| spellingShingle | Molecular identification of tick-borne hemoparasites in equines from Northwestern Colombia Yeison Agudelo-Ruíz Veterinaria PCR Babesia Anaplasma Ehrlichia Theileria Molecular identification of tick-borne hemoparasites in equines from Northwestern Colombia Yeison Agudelo-Ruíz Leidy Acevedo-Gutiérrez Andrés Montoya-Sanchéz Luis Paternina Veterinaria PCR Babesia Anaplasma Ehrlichia Theileria Objective. To detect and identify Anaplasmataceae agents and piroplasms in equines from the slaughterhouse “La Rinconada” at Rionegro municipality in Antioquia. Materials and methods. A descriptive cross-sectional study was carried out on equines selected by convenience during a period of 2015. Information about species, sex, age and origin of the animals. Whole blood was collected for DNA extraction procedure, and a PCR targeting a 360bp of Anaplasmataceae 16S ribosomal gene and 450bp of 18S ribosomal gene of Piroplasm were used for detection. PCR amplicons selected were submitted to direct sequencing for identification of hemoparasites through genetic analysis Results.135 equine samples from Antioquia, Cordoba y Sucre were analyzed. 78% were horses, 16% were donkeys and 6% were mules. Anaplasmataceae agents were not detected in any sample, meanwhile 13% were positive to piroplasm PCR. Sequence analysis reveals the circulation of Theileria equi in northwestern Colombia. Conclusions. This work presents the first molecular evidence of at least three genotypes of T. equi in equines of northwestern Colombia. 2017 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69353273003 https://www.redalyc.org/journal/693/69353273003/ https://www.redalyc.org/journal/693/69353273003/html/ https://www.redalyc.org/journal/693/69353273003/69353273003.epub https://www.redalyc.org/journal/693/69353273003/movil en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Vol.22 |
| title | Molecular identification of tick-borne hemoparasites in equines from Northwestern Colombia |
| topic | Veterinaria PCR Babesia Anaplasma Ehrlichia Theileria |
| url | https://www.redalyc.org/articulo.oa?id=69353273003 https://www.redalyc.org/journal/693/69353273003/ https://www.redalyc.org/journal/693/69353273003/html/ https://www.redalyc.org/journal/693/69353273003/69353273003.epub https://www.redalyc.org/journal/693/69353273003/movil |