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| Format: | Artículo científico |
| Language: | en |
| Published: |
Sociedade Brasileira de Proteção Radiológica - SBPR
2019
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| Online Access: | https://www.redalyc.org/articulo.oa?id=722280838005 |
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Table of Contents:
- FLOW CYTOMETRY BASED MICRONUCLEUS ASSAY FOR EVALUATION OF GENOTOXIC POTENTIAL OF 2-ACBs IN HEPATIC CELLS HepG2 Angélica B. Barbezan Carla J. B. Santos Luma R. Carvalho Daniel P. Vieira Glaucia M. M. Santelli Anna L. C. H. Villavicêncio Biología Food irradiation is approved for use in more than 60 countries for applications and purposes in a wide variety of foods, being an effective and safe method for preservation and long-term storage. 2- Alkylcyclobutanones (2-ACBs) are the only known radiolytic products generated from foods that contain fatty acids (Triglycerides) when irradiated. The acids analyzed in this study are palmitic and stearic, which when irradiated form 2-Dodecylcyclobutanones (2-dDCB) and 2-Tetradecylcyclobutanone (2-tDCB). Part of the 2-ACBs ingested is excreted through feces and part is deposited in adipose tissues. In vitro studies so far have been only in colon cells. The work used a human hepatoma cell line (HepG2) since the accumulation of fat in this organ is quite common. Micronucleus test was selected to evaluate possible genotoxic effects of 2-dDCB and 2-tDCB compounds when exposed to high concentrations (447, 1422 and 2235 μM) for 4 and 24 hours. Tests were performed in quadriplicates using flow cytometric analysis. None detectable genotoxic damage was observed after 4 hours of exposure to the compounds, and cytotoxic effects were only significant at the highest concentration (2235 μM) of 2-dDCB. After 24 hours of exposure, slight genotoxic damage was observed at all concentrations evaluated, and cytotoxic effects were only present when exposed to compound 2-tDCB. Although there is a genotoxic and cytotoxic effect in some of the situations tested, the two compounds predominantly induced proliferation reduction effects of this hepatic tumor cell line. 2019 artículo científico 2319-0612 https://www.redalyc.org/articulo.oa?id=722280838005 en http://www.redalyc.org/revista.oa?id=7222 Brazilian Journal of Radiation Sciences application/pdf Sociedade Brasileira de Proteção Radiológica - SBPR Brazilian Journal of Radiation Sciences (Brasil) Num.2A Vol.7