Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Sociedade Brasileira de Proteção Radiológica - SBPR
2019
|
| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=722280838025 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866582280131051520 |
|---|---|
| author | Ana Carolina de Araujo Bispo |
| author_facet | Ana Carolina de Araujo Bispo |
| contents | Development of an HPLC method for the radiochemical purity evaluation of [18F] fluoroestradiol Ana Carolina de Araujo Bispo Leonardo Tafas Constantino Nascimento Flávia Mesquita Costa Juliana Batista Silva Marcelo Mamede Biología 18F HPLC Fluoroestradiol Radiochemical Purity 18F-Fluoroestradiol ([18F]FES), an estrogen analog, is a radiopharmaceutical used in Positron Emission Tomography (PET) that allows evaluating the tumor cell receptor profile and the best therapy strategy, the staging, the prognosis and the response to therapy in several breast cancer cases. As there is not any pharmacopoeia’s monograph of [18F]FES to standardize its quality control criteria, this work presents a new HPLC’s method to perform the [18F]FES radiochemical purity. A liquid chromatograph was used with radioactivity and ultraviolet detectors. Three concentrations of fluoroestradiol standard solution were used along the test. Their retention time was compared to its relative radiolabelled analogue to confirm its identity. Several mobile phases with acetonitrile and two mobile phase flows were tested to optimize the runs. Peaks symmetry, retention time, theoretical plates and resolution were analyzed to choose the best conditions. The mean retention time of both standard Fluoroestradiol and [18F]FES solutions were the same, demonstrating that [ 18F]FES formulation did not interfere with [18F]FES analysis. The best conditions were 1.2 mL/min and isocratic 40% V/V acetonitrile in water, which gave [18F]FES peak resolution greater than 6 and symmetry factor of 1. Thus, the developed method is ready to be validated and implemented in [18F]FES quality control routine in CDTN/Brazil. 2019 artículo científico 2319-0612 https://www.redalyc.org/articulo.oa?id=722280838025 en http://www.redalyc.org/revista.oa?id=7222 Brazilian Journal of Radiation Sciences application/pdf Sociedade Brasileira de Proteção Radiológica - SBPR Brazilian Journal of Radiation Sciences (Brasil) Num.2A Vol.7 |
| format | Artículo científico |
| id | redalyc_722280838025 |
| language | en |
| publishDate | 2019 |
| publisher | Sociedade Brasileira de Proteção Radiológica - SBPR |
| spellingShingle | Development of an HPLC method for the radiochemical purity evaluation of [18F] fluoroestradiol Ana Carolina de Araujo Bispo Biología 18F HPLC Fluoroestradiol Radiochemical Purity Development of an HPLC method for the radiochemical purity evaluation of [18F] fluoroestradiol Ana Carolina de Araujo Bispo Leonardo Tafas Constantino Nascimento Flávia Mesquita Costa Juliana Batista Silva Marcelo Mamede Biología 18F HPLC Fluoroestradiol Radiochemical Purity 18F-Fluoroestradiol ([18F]FES), an estrogen analog, is a radiopharmaceutical used in Positron Emission Tomography (PET) that allows evaluating the tumor cell receptor profile and the best therapy strategy, the staging, the prognosis and the response to therapy in several breast cancer cases. As there is not any pharmacopoeia’s monograph of [18F]FES to standardize its quality control criteria, this work presents a new HPLC’s method to perform the [18F]FES radiochemical purity. A liquid chromatograph was used with radioactivity and ultraviolet detectors. Three concentrations of fluoroestradiol standard solution were used along the test. Their retention time was compared to its relative radiolabelled analogue to confirm its identity. Several mobile phases with acetonitrile and two mobile phase flows were tested to optimize the runs. Peaks symmetry, retention time, theoretical plates and resolution were analyzed to choose the best conditions. The mean retention time of both standard Fluoroestradiol and [18F]FES solutions were the same, demonstrating that [ 18F]FES formulation did not interfere with [18F]FES analysis. The best conditions were 1.2 mL/min and isocratic 40% V/V acetonitrile in water, which gave [18F]FES peak resolution greater than 6 and symmetry factor of 1. Thus, the developed method is ready to be validated and implemented in [18F]FES quality control routine in CDTN/Brazil. 2019 artículo científico 2319-0612 https://www.redalyc.org/articulo.oa?id=722280838025 en http://www.redalyc.org/revista.oa?id=7222 Brazilian Journal of Radiation Sciences application/pdf Sociedade Brasileira de Proteção Radiológica - SBPR Brazilian Journal of Radiation Sciences (Brasil) Num.2A Vol.7 |
| title | Development of an HPLC method for the radiochemical purity evaluation of [18F] fluoroestradiol |
| topic | Biología 18F HPLC Fluoroestradiol Radiochemical Purity |
| url | https://www.redalyc.org/articulo.oa?id=722280838025 |