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| Format: | Artículo científico |
| Sprache: | en |
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Sociedade Brasileira de Proteção Radiológica - SBPR
2019
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| Online-Zugang: | https://www.redalyc.org/articulo.oa?id=722280838051 |
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Inhaltsangabe:
- Metodological issues in protein and lipidic expressions in brain tissue exposed to Co 60 based on DESI/MALDI-MSI Matheus Figueiredo Soares Mingote Tarcísio P. R. Campos Rodinei Augusti Marcos N. Eberlin Biología DESI MALDI lipidomics radiation effects Ionization mass spectrometry The purpose of this work is to demonstrate important changes in the methodology for the identification of lipid and protein changes in the brain tissue induced by radiation. The objective was to analyze the methodology and investigate the viability of the generation of lipid / protein profiles of irradiated brain tissue; disregarding the results of the analyzes. Lipids and proteins are biomolecules necessary for numerous intracellular processes. Alterations in lipid and tissue protein profiles may indicate a radioinduced cellular response that can lead to neoplasms or neurodegenerative diseases such as Alzheimer's disease. DESI-MSI allows the generation of images according to each m / z that allow the identification and analytical quantification of lipids according to their spatial distribution in the tissue. MALDI-MSI is already a method used in the study of macromolecules as membrane peptides, structural, hormonal, neuromediators and immunological. Both processes have peculiarities for obtaining images, yet in the end they provide the molecular fingerprint with the image formed pixel by pixel in 2D. The produced image allows to associate the distribution of the lipids and proteins in the tissues with their identified chemical profile, allowing the verification of the radio induced changes produced by the reactive oxygen species (ROS). The production of the images was directly dependent on the rigorous execution of the methodological procedures. Numerous interferences can damage generations of the image and the analysis of proteins and lipids. In the near future, it will be possible to detect changes in the spatial distribution and types of lipids and proteins in the white and gray regions. 2019 artículo científico 2319-0612 https://www.redalyc.org/articulo.oa?id=722280838051 en http://www.redalyc.org/revista.oa?id=7222 Brazilian Journal of Radiation Sciences application/pdf Sociedade Brasileira de Proteção Radiológica - SBPR Brazilian Journal of Radiation Sciences (Brasil) Num.2A Vol.7