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| Format: | Artículo científico |
| Language: | en |
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Sociedad Mexicana de Nutrición y Tecnología de Alimentos
2005
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| Online Access: | https://www.redalyc.org/articulo.oa?id=72450102 |
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| _version_ | 1866811828078641152 |
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| author | C. M. Carvalho |
| author_facet | C. M. Carvalho |
| contents | Identification of honey yeast species based on RFLP analysis of the its region C. M. Carvalho M. L. F. Estevinho A. Choupina A. Rocha Química 8S RFLP ITS region Honey yeast identification In the present study, the restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene were used to identify a total of seven honey yeast species belonging to six different genera. Polymerase chain reaction (PCR) products of this rDNA region showed a high length variation for the different species. The size of the PCR products and the restriction patterns obtained with endonucleases HhaI, HaeIII and HinfI yielded a unique profile for each species, except for Zygoscharomyces mellis. The use of this molecular approach is proposed as a new rapid and easy method of routine honey yeast identification. 2005 artículo científico 1135-8122 https://www.redalyc.org/articulo.oa?id=72450102 en http://www.redalyc.org/revista.oa?id=724 Ciencia y Tecnología Alimentaria application/pdf Sociedad Mexicana de Nutrición y Tecnología de Alimentos Ciencia y Tecnología Alimentaria (México) Num.1 Vol.5 |
| format | Artículo científico |
| id | redalyc_72450102 |
| language | en |
| publishDate | 2005 |
| publisher | Sociedad Mexicana de Nutrición y Tecnología de Alimentos |
| spellingShingle | Identification of honey yeast species based on RFLP analysis of the its region C. M. Carvalho Química 8S RFLP ITS region Honey yeast identification Identification of honey yeast species based on RFLP analysis of the its region C. M. Carvalho M. L. F. Estevinho A. Choupina A. Rocha Química 8S RFLP ITS region Honey yeast identification In the present study, the restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene were used to identify a total of seven honey yeast species belonging to six different genera. Polymerase chain reaction (PCR) products of this rDNA region showed a high length variation for the different species. The size of the PCR products and the restriction patterns obtained with endonucleases HhaI, HaeIII and HinfI yielded a unique profile for each species, except for Zygoscharomyces mellis. The use of this molecular approach is proposed as a new rapid and easy method of routine honey yeast identification. 2005 artículo científico 1135-8122 https://www.redalyc.org/articulo.oa?id=72450102 en http://www.redalyc.org/revista.oa?id=724 Ciencia y Tecnología Alimentaria application/pdf Sociedad Mexicana de Nutrición y Tecnología de Alimentos Ciencia y Tecnología Alimentaria (México) Num.1 Vol.5 |
| title | Identification of honey yeast species based on RFLP analysis of the its region |
| topic | Química 8S RFLP ITS region Honey yeast identification |
| url | https://www.redalyc.org/articulo.oa?id=72450102 |