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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Sociedad Mexicana de Nutrición y Tecnología de Alimentos
2006
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| Accesso online: | https://www.redalyc.org/articulo.oa?id=72450207 |
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Sommario:
- pRS4: un vector de clonación idóneo para bacterias ácidolácticas de uso alimentario J. M. Mesas M. C. Rodríguez M. T. Alegre Química Lactic plasmids acid bacteria cloning vectors The ability of pRS4, a cryptic plasmid from Pediococcus pentosaceus, as a selective cloning vector for lactic acidbacteria has been analysed. The results indicate that pRS4C1, a cloning vector from pRS4 can stably transform Lactobacillus plantarum, Pediococcus acidilactici and P. pentosaceus (three lactic acid bacteria used as starters in the food industry) but it does not transform Enterococcus faecalis (a lactic acid bacteria which is undesirable due to its potential origin,mainly of faecal source). Further modifications of the pRS4C1, consisting in positional changes of the chloramphenicol resistance marker and/or single mutation into the initiation codon of the gene of the replication protein, does not generateexpression of pRS4 into E. faecalis, however the ability of these derivates to transform Lactobacillus and Pediococcus remains unaltered. These results indicate that pRS4 can be used as a selective cloning vector for genetic manipulation of useful lactic acid bacteria, without risk of horizontal transfer of genetic material to undesirable lactic acid bacteria that could coexist with the formers. 2006 artículo científico 1135-8122 https://www.redalyc.org/articulo.oa?id=72450207 en http://www.redalyc.org/revista.oa?id=724 Ciencia y Tecnología Alimentaria application/pdf Sociedad Mexicana de Nutrición y Tecnología de Alimentos Ciencia y Tecnología Alimentaria (México) Num.2 Vol.5