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| Format: | Artículo científico |
| Sprache: | es |
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Universidad San Francisco de Quito
2012
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| Online-Zugang: | https://www.redalyc.org/articulo.oa?id=726180833011 |
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- Efecto de la temperatura, medios de cultivo y reguladores de crecimiento en la germinación de embriones cigóticos de durazno (Prunus persica) var. Diamante Venancio Arahana Homero Arteaga Jose Tobar Viviana Jaramillo Maria de Lourdes Torres Multidisciplinaria (Ciencias Naturales y Exactas) Prunus persica stratification zygotic embryos growth regulators in vitro germination In this research, the effects of temperature, culture media and growth regulators on mature peach embryos (var. Diamante) were evaluated. Temperature had a significant influence on germination, as embryos cultured at 4◦C for 40 days and then moved to a temperature of 18◦C had a germination rate of 84 %, compared to embryos cultured directly at 18◦C, which had a germination rate of 50 %. Regarding the culture media, the highest germination rate was achieved using MS media with half the concentration of salts. Despite not having significant differences when adding growth regulators in the culture media, the addition of high concentrations of cytokinins (>1mg/L) and low concentrations of auxins (0.5mg/L) generated plants with a higher number of leaves. The best root development was obtained by the subculture of plants in MS media with 3mg/L of IBA for 16 days. The acclimatization of plants was successful, with a survival rate of 80 % in plants that germinated from embryos cultured at 4◦C for 40 days. This standardized protocol was also tested in the germination of var. Diamante x var. Florida peach hybrids, with a germination rate of 61 %, proving to be a potentially efficient method for peach propagation in crop improvement programs. 2012 artículo científico 1390-5384 https://www.redalyc.org/articulo.oa?id=726180833011 es http://www.redalyc.org/revista.oa?id=7261 Avances en Ciencias e Ingenierías application/pdf Universidad San Francisco de Quito Avances en Ciencias e Ingenierías (Ecuador) Num.2 Vol.4