First report of Porcine Circovirus 2 (PCV2) circulation in Ecuador
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| Format: | Artículo científico |
| Language: | en |
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Universidad San Francisco de Quito
2013
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| _version_ | 1876459531242831872 |
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| author | Estefanía Bermeo |
| author_facet | Estefanía Bermeo |
| contents | First report of Porcine Circovirus 2 (PCV2) circulation in Ecuador Estefanía Bermeo Verónica Barragán Alejandro Torres Alfredo Acosta Gabriel Trueba Multidisciplinaria (Ciencias Naturales y Exactas) PCR DNA Ecuador Porcine Circovirus Type 2 This report describes the first detection of Porcine Circovirus Type 2 (PCV2) from pigs in Ecuador. Samples were collected from 162 animals from November 2010 to March 2011: 25 sick animals from commercial swine herds and 137 from slaughterhouses. To detect the virus we used polymerase chain reaction (PCR) and two primer set. Our procedure revealed PCV2 DNA in 24 of 25 clinically affected pigs from farms and 77 of 137 pigs from slaughterhouses. The nucleotide sequence analysis of nine amplicons confirmed the PCV2 detection with at least a 95% homology compared with other PCV2 found in the GenBank database. Additionally, microscopic lesions in tissues of clinically affected pigs suggested porcine circovirus infection. 2013 artículo científico 1390-5384 https://www.redalyc.org/articulo.oa?id=726180834003 en http://www.redalyc.org/revista.oa?id=7261 Avances en Ciencias e Ingenierías application/pdf Universidad San Francisco de Quito Avances en Ciencias e Ingenierías (Ecuador) Num.1 Vol.5 |
| format | Artículo científico |
| id | redalyc_726180834003 |
| institution | Redalyc |
| language | en |
| publishDate | 2013 |
| publisher | Universidad San Francisco de Quito |
| spellingShingle | First report of Porcine Circovirus 2 (PCV2) circulation in Ecuador Estefanía Bermeo Multidisciplinaria (Ciencias Naturales y Exactas) PCR DNA Ecuador Porcine Circovirus Type 2 First report of Porcine Circovirus 2 (PCV2) circulation in Ecuador Estefanía Bermeo Verónica Barragán Alejandro Torres Alfredo Acosta Gabriel Trueba Multidisciplinaria (Ciencias Naturales y Exactas) PCR DNA Ecuador Porcine Circovirus Type 2 This report describes the first detection of Porcine Circovirus Type 2 (PCV2) from pigs in Ecuador. Samples were collected from 162 animals from November 2010 to March 2011: 25 sick animals from commercial swine herds and 137 from slaughterhouses. To detect the virus we used polymerase chain reaction (PCR) and two primer set. Our procedure revealed PCV2 DNA in 24 of 25 clinically affected pigs from farms and 77 of 137 pigs from slaughterhouses. The nucleotide sequence analysis of nine amplicons confirmed the PCV2 detection with at least a 95% homology compared with other PCV2 found in the GenBank database. Additionally, microscopic lesions in tissues of clinically affected pigs suggested porcine circovirus infection. 2013 artículo científico 1390-5384 https://www.redalyc.org/articulo.oa?id=726180834003 en http://www.redalyc.org/revista.oa?id=7261 Avances en Ciencias e Ingenierías application/pdf Universidad San Francisco de Quito Avances en Ciencias e Ingenierías (Ecuador) Num.1 Vol.5 |
| title | First report of Porcine Circovirus 2 (PCV2) circulation in Ecuador |
| topic | Multidisciplinaria (Ciencias Naturales y Exactas) PCR DNA Ecuador Porcine Circovirus Type 2 |
| url | https://www.redalyc.org/articulo.oa?id=726180834003 |