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| Format: | Artículo científico |
| Language: | en |
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Universidad de Guadalajara
2006
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| Online Access: | https://www.redalyc.org/articulo.oa?id=73000410 |
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Table of Contents:
- Attachment evaluation of embryonic stem cells on a pau-coated non-woven fabric: a potential source for bioartificial assist devices Jorge David Rivas Carrillo Chen Yong Tanaka Noriaki Alejandro Soto Gutiérrez Nalu Navarro Alvarez Naoya Kobayashi Multidisciplinarias (Ciencias Sociales) bio fiber and PAU like cells attachment Embryonic stem (ES) cells have been recently studied as an attractive source of bioartificial organs. The proliferationand differentiation of ES cells on such device should be considered. We have currently developed a hollow fiber Poly-aminourethan-coated unwoven PFTE fabric-typed (PAU-coated PTFE) bioartificial liver module. Here, we have evaluated theattachment capacity of ES cells, embryonic bodies and the functional capacity of hepatocyte-like cells derived from mouseembryonic stem cells on nonwoven PTFE fabric. To efficiently produce a large number of hepatocyte like cells from mouse EScells, we formed embryonic bodies and plated on matrigel in the presence of fibroblast growth factor-2, and the deleted form ofhepatocyte growth factor as well of dymetil sulfoxide and dexametasone. Cellular adherence and morphology on PAU-coatedunwoven PTFE fabric was regularly assessed by a scanning electron microscope (SEM). For hepatocyte differentiation, geneexpression of albumin was examined. To test the function of these hepatocytes in vitro we evaluated the metabolizing capacityusing ammonia and lidocaine and found that the cells cultured on PTFE expressed and secreted better quantities of albumin andsignificantly better metabolizing capacities. In the present work we have demonstrated the successful attachment of mouse EScells on PAU-coated unwoven PTFE fabric and subsequent hepatic differentiation. Thus, culture and subsequent differentiation ofES cells on PAU-coated unwoven PTFE fabric could be a useful new scaffolding biomaterial towards to develop bio-artificialliver and a new model for drug screening 2006 artículo científico 1665-5745 https://www.redalyc.org/articulo.oa?id=73000410 en http://www.redalyc.org/revista.oa?id=730 e-Gnosis application/pdf Universidad de Guadalajara e-Gnosis (México) Num.4