Análisis de rutas metabólicas en Pseudomonas aeruginosa para la producción de polihidroxialcanoatos a partir de glucosa usando modos elementales
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| Format: | Artículo científico |
| Langue: | en |
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Universidad de Guadalajara
2006
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| _version_ | 1876441256094072832 |
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| author | Oscar Rojas R. |
| author_facet | Oscar Rojas R. |
| contents | Análisis de rutas metabólicas en Pseudomonas aeruginosa para la producción de polihidroxialcanoatos a partir de glucosa usando modos elementales Oscar Rojas R. Juan Villafaña R. Jesús Nungaray A. Orfil González R. Multidisciplinarias (Ciencias Sociales) reducing power Fluxes distribution Entner Doudoroff Pathway The polyhydroxyalkanoates (PHAs) are metabolites accumulated as energy and carbon sources in Pseudomonasaeruginosa under nitrogen limited conditions. In this work, the metabolic capability directed to polyhydroxyalkanoates (PHAs)biosynthesis in P. aeruginosa is evaluated through a flux distribution analysis as a result of the calculus of the Elementary Modesdetermined from the reconstruction of its metabolic network. 188 EMs were obtained as a result of the metabolic networkflexibility. Considering that the products derived by the extracellular oxidative pathway (gluconic and 2-keto-gluconic acids) arenot cometabolized along with the glucose, the number of EMs to analyze is reduced to 38. The reduced set of EMs contains themetabolic flux distributions with the highest yields calculated to biomass (0.102 g of biomass/mmol of glucose) and PHAsproduction (0.397 mmol of PHAs/mmol of glucose). The accumulation process is described under two metabolic scenarios, withand without cell growth. Under non growth condition, two maximum yields are achieved, 0.198 and 0.397 mmol of PHAs/ mmolof glucose, respectively. The highest yield is obtained when the Entner Doudoroff pathway is active instead of Pentose PhosphatePathway, because less carbon is lost as CO2 and there is a suitable distribution of the reducing power. In the case of simultaneousgrowth and accumulation condition the maximum PHAs yield is 0.355 mmol of PHAs/mmol of glucose and a maximum growthrate of 0.024 h-1. The distribution of Acetyl-CoA into the tricarboxylic acids cycle and the novo biosynthesis of fatty acidsdetermines the limited reaction step in the synthesis of PHAs. As a consequence of the increment of Acetyl-CoA flux toward thenovo biosynthesis of fatty acids and a reduction of this flux to the tricarboxylic acids cycle, the synthesis of PHAs is favored,diminishing the metabolic flux torward the biosynthetic precursors and causing a decrease in the growth rate 2006 artículo científico 1665-5745 https://www.redalyc.org/articulo.oa?id=73000412 en http://www.redalyc.org/revista.oa?id=730 e-Gnosis application/pdf Universidad de Guadalajara e-Gnosis (México) Num.4 |
| format | Artículo científico |
| id | redalyc_73000412 |
| institution | Redalyc |
| language | en |
| publishDate | 2006 |
| publisher | Universidad de Guadalajara |
| spellingShingle | Análisis de rutas metabólicas en Pseudomonas aeruginosa para la producción de polihidroxialcanoatos a partir de glucosa usando modos elementales Oscar Rojas R. Multidisciplinarias (Ciencias Sociales) reducing power Fluxes distribution Entner Doudoroff Pathway Análisis de rutas metabólicas en Pseudomonas aeruginosa para la producción de polihidroxialcanoatos a partir de glucosa usando modos elementales Oscar Rojas R. Juan Villafaña R. Jesús Nungaray A. Orfil González R. Multidisciplinarias (Ciencias Sociales) reducing power Fluxes distribution Entner Doudoroff Pathway The polyhydroxyalkanoates (PHAs) are metabolites accumulated as energy and carbon sources in Pseudomonasaeruginosa under nitrogen limited conditions. In this work, the metabolic capability directed to polyhydroxyalkanoates (PHAs)biosynthesis in P. aeruginosa is evaluated through a flux distribution analysis as a result of the calculus of the Elementary Modesdetermined from the reconstruction of its metabolic network. 188 EMs were obtained as a result of the metabolic networkflexibility. Considering that the products derived by the extracellular oxidative pathway (gluconic and 2-keto-gluconic acids) arenot cometabolized along with the glucose, the number of EMs to analyze is reduced to 38. The reduced set of EMs contains themetabolic flux distributions with the highest yields calculated to biomass (0.102 g of biomass/mmol of glucose) and PHAsproduction (0.397 mmol of PHAs/mmol of glucose). The accumulation process is described under two metabolic scenarios, withand without cell growth. Under non growth condition, two maximum yields are achieved, 0.198 and 0.397 mmol of PHAs/ mmolof glucose, respectively. The highest yield is obtained when the Entner Doudoroff pathway is active instead of Pentose PhosphatePathway, because less carbon is lost as CO2 and there is a suitable distribution of the reducing power. In the case of simultaneousgrowth and accumulation condition the maximum PHAs yield is 0.355 mmol of PHAs/mmol of glucose and a maximum growthrate of 0.024 h-1. The distribution of Acetyl-CoA into the tricarboxylic acids cycle and the novo biosynthesis of fatty acidsdetermines the limited reaction step in the synthesis of PHAs. As a consequence of the increment of Acetyl-CoA flux toward thenovo biosynthesis of fatty acids and a reduction of this flux to the tricarboxylic acids cycle, the synthesis of PHAs is favored,diminishing the metabolic flux torward the biosynthetic precursors and causing a decrease in the growth rate 2006 artículo científico 1665-5745 https://www.redalyc.org/articulo.oa?id=73000412 en http://www.redalyc.org/revista.oa?id=730 e-Gnosis application/pdf Universidad de Guadalajara e-Gnosis (México) Num.4 |
| title | Análisis de rutas metabólicas en Pseudomonas aeruginosa para la producción de polihidroxialcanoatos a partir de glucosa usando modos elementales |
| topic | Multidisciplinarias (Ciencias Sociales) reducing power Fluxes distribution Entner Doudoroff Pathway |
| url | https://www.redalyc.org/articulo.oa?id=73000412 |