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| Format: | Artículo científico |
| Language: | en |
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Universidad Libre
2010
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| Online Access: | https://www.redalyc.org/articulo.oa?id=757979590010 |
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Table of Contents:
- Obtaining of Reducing Sugars from Kikuyu Grass (Pennisetum Clandestinum) Andrés Felipe López Vásquez Gabriel Alberto Ortegón Rey Fredy Antonio Robles Rodríguez Ingeniería Hydrolysis Kikuyu grass Reducing sugars The production of reducing sugars by acid and enzymatic hydrolysis of Kikuyu grass was performed in order to study reducing sugar production. The study involved hydrolysis of treated grass by immersed in water at environmental temperatures. Acid hydrolysis was carried out through reflux boiling with different concentrations of diluted sulphuric acid (2%, 4%, 6% v/v) with in a liquid/ solid ratio of 30/1, while enzymatic hydrolysis was carried out with stirring in a water/enzyme (celluclast 1,5 LFG) solution. Both procedures were developed to different reaction times (2, 4 and 6 hrs). Reducing sugars concentrations were determined by UV-Vis spectrophotometry using the 3,5 dinitrosalisilic acid (DNS) method. Also, Benedict qualitative test confirmed reducing sugars presence in the samples. The greatest reducing sugars concentration was 2.5 g/l when using grass with aqueous treatment, with a 4 % sulfuric acid solution and 6 hours of reaction time. In general, results showed that greatest reducing sugars concentration were to high acid concentrations and reaction times. 2010 artículo científico 2619-6581 https://www.redalyc.org/articulo.oa?id=757979590010 en http://www.redalyc.org/revista.oa?id=7579 Avances application/pdf Universidad Libre Avances (Colombia) Num.13 Vol.1