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Detalles Bibliográficos
Autor principal:	Yeimy Alexandra Pinzón Gutiérrez
Formato:	Artículo científico
Lenguaje:	es
Publicado:	Universidad Nacional de Colombia 2013
Materias:	Biología α ) Ñame pudrición antracnosis Fusarium oxysporum
Acceso en línea:	https://www.redalyc.org/articulo.oa?id=77628609006
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

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Diagnóstico molecular diferencial de Colletotrichum gloeosporioides y Fusarium oxysporum en ñame (Dioscorea sp.) Yeimy Alexandra Pinzón Gutiérrez Silvia Lizette Bustamante Gustavo Buitrago Hurtado Biología α ) Ñame pudrición antracnosis Fusarium oxysporum Las enfermedades de origen fúngico son responsables de las mayores pérdidas reportadas en los cultivos de ñame colom - bianos, entre ellas la antracnosis causada por Colletotrichum spp. se destaca por ser la más devastadora. En los últimos años, hongos con baja prevalencia pero con alto poder de diseminación como Fusarium spp., han ampliado su presencia en los cultivos de ñame favoreciendo el desarrollo de enfermedades como la pudrición del tubérculo en postcosecha. A pesar de la importancia del cultivo de ñame en las regiones de la Costa Atlántica colombiana, el Pacífico y la Amazonia, este es considerado un cultivo huérfano en razón a que son pocos los esfuerzos que en investigación se realizan a nivel mundial y particularmente en Colombia. Con el propósito de aportar al diagnóstico correcto y oportuno de fitopatógenos que afec - tan el cultivo de ñame, se obtuvieron aislamientos fúngicos a partir de hojas con manchas necróticas. De los aislamientos obtenidos, cinco presentaron morfología propia del género Colletotrichum y tres de Fusarium . La identidad de las especies implicadas se determinó por secuenciación de los ITS del ADNr, correspondiendo a Colletotrichum gloeosporioides y Fu - sarium oxysporum . La evaluación de marcadores moleculares para la detección de forma diferencial y simultánea de estos patógenos, permitió seleccionar la amplificación del factor de elongación alpha (EF- α ) como la mejor prueba de diagnosis. Las pruebas de patogenicidad confirmaron la capacidad de los aislados de C. gloeosporioides para causar sintomatología de antracnosis en foliolos y de los aislados de F. oxysporum para desencadenar pudrición en tubérculo, la presencia de este último en hojas se podría asociar a su fácil y rápida dispersión conidial, a su acentuada incidencia en suelo que es su hábitat natural y a su prolongada sobrevivencia. 2013 artículo científico 0123-3475 https://www.redalyc.org/articulo.oa?id=77628609006 es http://www.redalyc.org/revista.oa?id=776 Revista Colombiana de Biotecnología application/pdf Universidad Nacional de Colombia Revista Colombiana de Biotecnología (Colombia) Num.1 Vol.XV

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// Anecdotal testing Today and Yesterday seems to indicate destroy() // is called by the garbage collector and everything is good. // Something to keep in mind though. return 0; } /** * Write function that is called when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ public function write($sessId, $data): bool { if ($this->writesDisabled) { return true; } return $this->saveSession($sessId, $data); } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ abstract protected function saveSession($sessId, $data): bool; }

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