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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Universidad Nacional de Colombia
2016
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| Accesso online: | https://www.redalyc.org/articulo.oa?id=77645907009 https://www.redalyc.org/journal/776/77645907009/ https://www.redalyc.org/journal/776/77645907009/html/ https://www.redalyc.org/journal/776/77645907009/77645907009.epub https://www.redalyc.org/journal/776/77645907009/movil |
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Sommario:
- Assessment three constitutive promoters for GUS expression in rice (Oryza sativa L., var. J-104) Maylin Pérez Bernal Daymí Abreu Remedios Onel Valdivia Pérez Magalis Delgado Rigo Raúl Armas Ramos Biología CaMV 35S ubiquitine chimerical A9 promoter This work analyzed the constitutive expression of the ß-Glucuronidase (GUS) reporter gene fused to three promoters: the cauliflower mosaic virus (CaMV) 35S, the chimerical A9 promoter which contains rice Act1, and the Ubiquitine-1 promoter from maize. The activity of the promoters was qualitative and quantitatively obtained in different tissues and various growth stages of rice plants (cv J-104) transformed by biolistic. All the promoters were found to be active, with distinct patterns of relative activity in leaves, stem and roots from in vitro and ex vitro plants, and in plants of T1 progeny. The chimerical A9 promoter increased significantly levels of GUS expression in all the tissues and at all growth stages of the plants. 2016 artículo científico 0123-3475 https://www.redalyc.org/articulo.oa?id=77645907009 https://www.redalyc.org/journal/776/77645907009/ https://www.redalyc.org/journal/776/77645907009/html/ https://www.redalyc.org/journal/776/77645907009/77645907009.epub https://www.redalyc.org/journal/776/77645907009/movil en http://www.redalyc.org/revista.oa?id=776 Revista Colombiana de Biotecnología application/pdf Universidad Nacional de Colombia Revista Colombiana de Biotecnología (Colombia) Num.1 Vol.XVIII